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MTK4a_003

Plasmid

#123817

Purpose

Encodes Nuclear import sequence as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

NLS

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEFIRES NQO1 Y128V

Plasmid

#61733

Purpose

Expresses human Y128V NQO1

Depositor

Yosef Shaul

Article

Asher et al Genes Dev. 2005 Feb 1;19(3):316-21.

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Tags

None

Expression

Mammalian

Mutation

Y128V

Promoter

Ef1a

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pBW809_pCAG-PV6-F3-mCherry-BGHpA

Plasmid

#87585

Purpose

PV6 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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MXS_tTA2

Plasmid

#62429

Purpose

MXS_chaining vector with rTA2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tetracycline transactivator

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pMaCTag-18

Plasmid

#119997

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mKeima8.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKeima8.5

Use

Pcr template

Tags

mKeima8.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHu6-gRNA-NT1

Plasmid

#81203

Purpose

hU6 expression of gRNA NT1

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA NT1

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pGEM-gate

Plasmid

#113758

Purpose

Gateway destination vector only containing the Gateway cassette and pGEM-T Easy backbone. This can be used with Gateway-compatible Homology-Directed Repair.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

gateway cassette

Use

Gateway destination vector

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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MTK3_002

Plasmid

#123719

Purpose

Encodes bsr (blasticidin-S deaminase) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Blasticydin Resistance

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBW820_pCAG-pExpr-FULL-ADDER

Plasmid

#87552

Purpose

Encodes full adder logic in response to Cre, Flp and VCre recombinases

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP, mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pPD551 ZF92x6-Compact_EYFP

Plasmid

#138865

Purpose

EYFP reporter vector for the ZF92x6-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF92x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF92x6-Compact

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pPD392 VPR-ZF92

Plasmid

#138841

Purpose

Constitutive expression of VPR-ZF92 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-ZF92

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pH2B-TagRFP675-N1

Plasmid

#44279

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

Insert

TagRFP675

Tags

Histone 2B

Expression

Mammalian

Mutation

M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pAG186 NID1 3'UTR

Plasmid

#12016

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N19 3'UTR (NID1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pPD289 ZF43x5-Compact_EYFP

Plasmid

#138900

Purpose

EYFP reporter vector for the ZF43x5-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x5-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x5-Compact

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pPD287 ZF43x3-Compact_EYFP

Plasmid

#138898

Purpose

EYFP reporter vector for the ZF43x3-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x3-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x3-Compact

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pPD286 ZF43x2-Compact_EYFP

Plasmid

#138897

Purpose

EYFP reporter vector for the ZF43x2-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x2-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x2-Compact

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pMaCTag-01

Plasmid

#119980

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with moxBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxBFP

Use

Pcr template

Tags

moxBFP

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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ARF17 pBluescript

Plasmid

#12068

Depositor

David Bartel

Article

Mallory et al Plant Cell. 2005 May . 17(5):1360-75.

Insert

ARF17 (ARF17 Mustard Weed)

Expression

Bacterial

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pEGFP(C1)-NIPP1/RKTK

Plasmid

#44222

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Article

Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Insert

NIPP1 (PPP1R8 Human)

Tags

GFP

Expression

Mammalian

Mutation

V201K and F203K

Promoter

CMV

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
MTK2_016

Plasmid

#123708

Purpose

Encodes truncated pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV-A

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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