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pAAV-Ef1a-DO-mCherry-WPRE-pA

Plasmid

#37119

Purpose

Expresses mCherry in cells lacking Cre (Cre-Off)

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

mCherry

Use

AAV and Cre/Lox; Cre-off

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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PKC zeta dNPS

Plasmid

#21250

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC zeta (Prkcz Rat)

Tags

HA

Expression

Mammalian

Mutation

delta 1-203

Available Since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
pLT 651

Plasmid

#59998

Purpose

Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny

Depositor

Lisa Timmons

Article

Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(

Inserts

klp-16 (klp-16 Nematode)
him-8 (him-8 Nematode)

Expression

Bacterial

Mutation

partial genomic

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV4Neo-murine IL-17RA-HA

Plasmid

#46859

Purpose

expresses mouse IL-17RA

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

IL-17RA (Il17ra Mouse)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
YAP1-Y357F-V5 in pLX304

Plasmid

#42556

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Y357F

Promoter

CMV

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only
mcherry Beta 1

Plasmid

#36111

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein beta subunit B1 (GNB1 Bovine)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
peCALWY-6

Plasmid

#22238

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY6 Zn(II) sensor (ATOX1 Human, GFP's)

Expression

Mammalian

Mutation

C416S mutation Identical to peCALWY-4, but linke…

Available Since

Jan. 5, 2010

Availability

Academic Institutions and Nonprofits only
peCALWY-5

Plasmid

#22237

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-5 Zn(II) sensor (ATOX1 Human, GFP's)

Expression

Mammalian

Mutation

C416S mutation identical to peCALWY-4, but linke…

Available Since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
pSG424 Smad2 (Gal4-Smad2)

Plasmid

#14932

Depositor

Joan Massague

Article

Liu et al Genes Dev. 1997 Dec 1. 11(23):3157-67.

Insert

Smad2 (SMAD2 Human)

Tags

Gal4

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
HA-HIF2α P405A/P531A/N847A-pBabe-Puro

Plasmid

#25956

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Oct. 19, 2010

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

Plasmid

#208046

Purpose

Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

RNF4 (RNF4 Human)

Use

Lentiviral

Tags

BirAopt

Promoter

EFS

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pTN4

Plasmid

#174212

Purpose

Donor plasmid for introducing Peft-3::AtTIR1(F79G)::mRuby at the ttTi5605 locus

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Inserts

AtTIR1(F79G)::mRuby (TIR1 Nematode)
GFP

Use

CRISPR and Cre/Lox

Tags

mRuby

Expression

Worm

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pmKate2-MS2coatProtein

Plasmid

#103833

Purpose

Live cell reader for MS2 loop RNA production

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

mKate2-MS2 coat protein (cp )

Expression

Mammalian

Mutation

MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-flag-hKDM4A(H188A)-polyA

Plasmid

#101052

Purpose

in vitro transcription (T7 promoter)

Depositor

Yi Zhang

Article

Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

Insert

KDM4A (KDM4A Human)

Tags

Flag

Expression

Mammalian

Promoter

T7

Available Since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-AT1R-APEX

Plasmid

#96847

Purpose

For expression of AT1R-APEX in mammalian cells

Depositor

Andrew Kruse

Article

Paek et al Cell. 2017 Apr 6;169(2):338-349.e11. doi

Insert

AT1R-APEX (AGTR1 Human)

Expression

Mammalian

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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Rlucrarr3-Flash1

Plasmid

#74129

Purpose

FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of Arrestin3.

Depositor

Louis Luttrell

Article

Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

Insert

Arrestin3

Tags

Renilla luciferase (rLuc)

Expression

Mammalian

Mutation

Insertion of FlAsH motif (CCPGCC) following amino…

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 3xFlag-p38alpha

Plasmid

#47575

Purpose

Bacterial expression plasmid for GST-3xFlag-tagged p38alpha

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

p38alpha (Mapk14 Mouse)

Tags

3xFlag and GST

Expression

Bacterial

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
2XMyc-LRRK2-K1347A

Plasmid

#25365

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Lysine 1347 mutated to Alanine

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-CaMKIIbeta(A303R)

Plasmid

#21224

Depositor

Tobias Meyer

Article

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CaMKIIbeta(A303R) (Camk2b Rat)

Tags

GFP

Expression

Mammalian

Mutation

CaMKIIbeta(A303R) (Lab plasmid CF 0002)

Available Since

July 29, 2009

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_F753A_6xH

Plasmid

#185710

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed F753 to A

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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