We narrowed to 6,411 results for: kit

1. pMXs-IP-EGFP-mAtg5

   Plasmid

   #38196

   Depositor

   Noboru Mizushima

   Article

   Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

   Insert

   autophagy related genes 5 (Atg5 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-IP-EGFP-LC3

   Plasmid

   #38195

   Depositor

   Noboru Mizushima

   Article

   Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-IP GFP-Omp25

   Plasmid

   #38249

   Depositor

   Noboru Mizushima

   Article

   Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

   Insert

   Omp25 (Synj2bp Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   plasmid contains AA109-145 of rat Omp25/Synj2bp

   Promoter

   Available since

   Sept. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)

   Plasmid

   #194222

   Purpose

   Mammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptors

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   SynNotch-GAL4 DBD-VP64 AD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xFLAG-dCas9/pMXs-IG

   Plasmid

   #51258

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-GLIS1 (FLJ33315SAAN)

   Plasmid

   #33274

   Purpose

   Retroviral expression of canonical human GLIS1

   Depositor

   Shinya Yamanaka

   Article

   Maekawa et al Nature. 2011 . 474(7350):225-9.

   Insert

   GLIS1, Gateway casette A (GLIS1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPV-H1-ccdB-mEF1α-RiH

   Plasmid

   #100598

   Purpose

   piggyBac vector for sgRNA cloning

   Depositor

   Akitsu Hotta

   Article

   Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

   Type

   Empty backbone

   Use

   Piggybac vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human H1 PolIII promoter, human FTH1 promoter, mo…

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-puro HA-Atg14*

   Plasmid

   #38265

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

   Insert

   autophagy related genes 14 (ATG14 Human)
   

   Use

   Retroviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   changed A618T, A621T, C624A and T627C for siRNA r…

   Promoter

   Available since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pMXs-puro GFP-UVRAG

   Plasmid

   #38267

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

   Insert

   UV radiation resistance-associated gene protein (UVRAG Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pLenti_dCas9-2xAM_hIRF-1

    Plasmid

    #92221

    Purpose

    Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Inserts

    Sp-dCas9-2xAM tag
    gRNA targeting human IRF-1 promoter
    

    Use

    CRISPR and Lentiviral

    Tags

    2xAM tag

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    CBh and U6

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1BR9-AtWUSSTOP

    Plasmid

    #202053

    Purpose

    E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    AtWUS E. Coli Codon Optimized (WUS Mustard Weed)
    

    Use

    Tags

    6xHis and GFP11

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)

    Plasmid

    #204619

    Purpose

    Expression vector of Cascade and Cas3. Puromycin selectable.

    Depositor

    Akitsu Hotta

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cas7, Cas5, Cas8, Cas11, Cas6, and Cas3
    

    Use

    CRISPR

    Tags

    Each protein has C- and N-terminal SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-Cas9-T2A-GFP

    Plasmid

    #63155

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9-T2A-GFP
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2pA2-ubb:secHsa.ANNEXINV-mVenus, cryaa:RFP

    Plasmid

    #92388

    Purpose

    Cell death reporter

    Depositor

    Nicholas Cole

    Article

    Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

    Insert

    ANNEXINV (ANXA5 Human)
    

    Use

    Zebrafish expression

    Tags

    mVenus

    Expression

    Mutation

    Promoter

    ubb

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-IP-EGFP-hAtg13

    Plasmid

    #38191

    Depositor

    Noboru Mizushima

    Article

    Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

    Insert

    autophagy-related genes 13 (ATG13 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pMXs-hLIN28A

    Plasmid

    #47902

    Purpose

    Expresses human LIN28A in mammalian cells using retrovirus system.

    Depositor

    Shinya Yamanaka

    Article

    Tanabe et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    human LIN28A (LIN28A Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMXs-hFOXH1GR

    Plasmid

    #59311

    Purpose

    Forced expression of human FOXH1GR

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

    Insert

    FOXH1 (FOXH1 Human)
    

    Use

    Retroviral

    Tags

    cGR - hormone binding domain of glucocorticoid re…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19-G9WGU4

    Plasmid

    #103112

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    G9WGU4(Cas9 coding gene from Oenococcus kitaharae DSM 17330)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p5E-1.7kbfabp6

    Plasmid

    #159087

    Purpose

    p5E with promoter sequence 1.7kb upstream of zebrafish fabp6 gene

    Depositor

    John Rawls

    Article

    Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

    Insert

    1.7kb fabp6 promoter (fabp6 Zebrafish)
    

    Use

    Promoter fragment 5' entry vector for tol2 c…

    Tags

    Expression

    Mutation

    Promoter

    1.7kb fabp6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-DMD-Donor

    Plasmid

    #60605

    Purpose

    Knock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    Hygromycin resistant gene
    

    Use

    Knock-in donor vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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