We narrowed to 7,705 results for: ALP

1. pRP(Exp)-CMV> ORF_924bp (Azgp1):dTomato:IRES:Puro

   Plasmid

   #110831

   Purpose

   Expresses both zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter, where the fluorescent dT reporter is fused with the ZAG protein.

   Depositor

   Ande Satyanarayana

   Article

   Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

   Insert

   Azgp1 (Azgp1 Mouse)
   

   Use

   Non-viral gene expression vector

   Tags

   dTomato

   Promoter

   CMV

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PET11a-Af2sir2

   Plasmid

   #8383

   Depositor

   Cynthia Wolberger

   Article

   Avalos et al Mol Cell 2002 Sep;10(3):523-35.

   Insert

   Sir2-Af2 full length (SIRT2, Sir2.2, SIRT1 Archaeoglobus Fulgidus)
   

   Expression

   Bacterial

   Mutation

   PET11a-Af2Sir2D103N

   Available Since

   Dec. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pONSY-Lifeact:mCherry

   Plasmid

   #111876

   Purpose

   This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   Lifeact fused to mCherry
   

   Use

   Capsaspora owczarzaki

   Tags

   mCherry

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pONSY-Venus

   Plasmid

   #111875

   Purpose

   This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.

   Depositor

   Inaki Ruiz-Trillo

   Article

   Parra-Acero et al Development. 2018 May 11. pii: dev.16210

   Insert

   Venus
   

   Use

   Capsaspora owczarzaki

   Tags

   Venus

   Promoter

   Elongation Factor 1 alpha (EF1a) from Capsaspora

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEN_TT S6K (E389-deltaCT)

   Plasmid

   #58507

   Purpose

   Gateway entry vector for an inducible HA-tagged rat S6K1-E389-deltaCT

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   Rps6kb1 (Rps6kb1 Rat)
   

   Use

   Entry vector

   Tags

   HA

   Mutation

   T389E, stop codon at amino acid 402

   Available Since

   Aug. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-EF1a-SwitchON_mRubyNLS-hChR2(H134R)-EYFP-NO_WPRE

   Plasmid

   #118279

   Purpose

   Expresses mRuby2 in the nucleus of Cre-negative cells, and ChR(H134R)-YFP in Cre-positive cells

   Depositor

   Dietmar Schmitz

   Article

   Wozny et al Front Cell Neurosci. 2018 Oct 2;12:337.

   Inserts

   mRuby2
   ChR2(H134R)
   

   Use

   AAV and Cre/Lox

   Tags

   SV40 NLS and YFP

   Promoter

   EF1 alpha

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PGEX-DDX21-D2+C

   Plasmid

   #179243

   Purpose

   protein purification in E. coli

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   DDX21-D2+C (DDX21 Human)
   

   Tags

   GST-flag

   Expression

   Bacterial

   Mutation

   aa396-782

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458_CEBPA_2

   Plasmid

   #86359

   Purpose

   Encodes gRNA for 3' target of human CEBPA

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against CEBPA (CEBPA Human)
   

   Use

   CRISPR

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCaSpeR4 cyto Grx1-roGFP2

   Plasmid

   #64999

   Purpose

   Expression of cytosolic Grx1-roGFP2 under the alphaTub84B promoter

   Depositor

   Tobias Dick

   Article

   Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Tags

   roGFP2

   Expression

   Insect

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. AtS/MAR10 insulator sequence (GB3458)

    Plasmid

    #160638

    Purpose

    pEGB3a1 AtS/MAR10

    Depositor

    Diego Orzaez

    Article

    Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

    Insert

    AtS/MAR10 insulator sequence
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPuroR-MCS-GAL4-MiniWhite

    Plasmid

    #165890

    Purpose

    Puromycin resistant GAL4 driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable Empty Enhancer Driver
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LITE1.0_pAAV_hSyn_CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid

    #48253

    Purpose

    LITE1.0 CRY2PHR fused to VP64 transcriptional activator domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression. See LITE2.0 for optimized LITE activators.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

    Insert

    CRY2PHR-NLS-VP64
    

    Use

    AAV; Tale

    Tags

    2A_GFP

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. PWPXLD-GFP-Numb-S276D

    Plasmid

    #133752

    Purpose

    Numb with mutation at serine 276 converted to aspartic acid cand tagged with GFP cloned into PWPXLD

    Depositor

    Chay Kuo

    Article

    Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

    Insert

    NUMB endocytic adaptor protein (Numb Mouse)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    serine 276 converted to aspartic acid

    Promoter

    EF1-alpha

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-TRIM24-C840W shRescue

    Plasmid

    #28137

    Depositor

    Michelle Barton

    Article

    Tsai et al Nature. 2010 Dec 16. 468(7326):927-32.

    Insert

    TRIM24 (TRIM24 Human)
    

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    C840Wmutated at aa 308 and 309 for shRNA rescue; …

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pCGN N-Ras 189L

    Plasmid

    #14724

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

    Insert

    N-ras (NRAS Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    189L

    Available Since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

16. Stat92e pPAcPL

    Plasmid

    #8713

    Depositor

    Jim Darnell

    Article

    Yan et al Cell 1996 Feb 9;84(3):421-30.

    Insert

    Stat92e (Stat92E Fly)
    

    Expression

    Insect

    Available Since

    July 20, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pJT45_GalL_nCDA1-BE3

    Plasmid

    #145038

    Purpose

    Expresses nCDA1-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMpGWB221

    Plasmid

    #68612

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Tags

    Modified EAR motif plant-specific repression doma…

    Expression

    Plant

    Promoter

    MpEF1alpha promoter

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFB-U6-Chrna7-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128342

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna7 (Chrna7 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCGN N-Ras 12D, 189L

    Plasmid

    #14726

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001;332:3-36. doi: 10.

    Insert

    N-ras (NRAS Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    12D, 189L

    Available Since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only