We narrowed to 10,238 results for: ada

1. qTAG-N-Blast-sTagRFP-TUBA1B

   Plasmid

   #207768

   Purpose

   Donor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a Blast-sTagRFP Cassette (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGEX 4t1 RARalpha (1-187)

   Plasmid

   #35558

   Depositor

   Jonathan Kurie, Harish Srinivas

   Article

   Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

   Insert

   RARalpha (RARA Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 1-187

   Available Since

   April 5, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pKK-FLAG-TEV

   Plasmid

   #105768

   Purpose

   Expression of your protein of interest in fusion with FLAG at the N-terminus. The tag is cleavable by TEV protease or enterokinase.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. S1 1029 CP ABEMax strategy 1

   Plasmid

   #135357

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   ABEMax-nSpyCas9 aa 1029
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDN-G1TGt

   Plasmid

   #44508

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
   tetR::yEGFP
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Expression

   Yeast

   Mutation

   Two tandem tet operators (2XtetO2) downstream of …

   Promoter

   PGal1-D12 promoter

   Available Since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV2_hSyn_Phobos_Citrine

   Plasmid

   #98216

   Purpose

   Blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.

   Depositor

   Simon Wiegert

   Article

   Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

   Insert

   Synthetic construct Phobos gene
   

   Use

   AAV

   Tags

   Citrine

   Expression

   Mammalian

   Mutation

   T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…

   Promoter

   human synapsin

   Available Since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSG5-FLAG-mEKLF

   Plasmid

   #67833

   Purpose

   Expression of FL-EKLF driven by SV40 promoter

   Depositor

   James Bieker

   Article

   Soni et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

   Insert

   EKLF (Klf1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Full-length mEKLF is aa 20-376; amino acid 19 is …

   Promoter

   SV40

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pMKO.1-shCcnb1

   Plasmid

   #162793

   Purpose

   Ccnb1 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Ccnb1 shRNA (Ccnb1 Mouse)
   

   Use

   RNAi and Retroviral

   Promoter

   U6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-hPMCA2xb

   Plasmid

   #47751

   Purpose

   mammalian expression of mCherry tagged hPMCA2, xb splice variant

   Depositor

   Emanuel Strehler

   Article

   Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi

   Insert

   PMCA2x/b (ATP2B2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   xb splice variant

   Promoter

   CMV

   Available Since

   Nov. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

10. pDisplay-GRAB_CRF0.5

    Plasmid

    #208654

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX 4T1 RARalpha (186-462)

    Plasmid

    #35559

    Depositor

    Jonathan Kurie, Harish Srinivas

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 186-462

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pJET-41-DExCon-antiGFPnanobody-mCherry-R11b

    Plasmid

    #179901

    Purpose

    Donor with homologous arms for Rab11b to knock in DExCon-antiGFPnanobody-mCherry module

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11B, member RAS oncogene family (RAB11B Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    antiGFPnanobody-mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    TRE3GS

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJET-07-mNeonGreen-R11a

    Plasmid

    #179890

    Purpose

    Donor with homologous arms for Rab11a to knock in mNeonGreen

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11A, member RAS oncogene family (RAB11A Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-6P-1 AUP1 292-410

    Plasmid

    #185331

    Purpose

    Bacterial expression of AUP1 with GST fusion protein for binding assays

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 292-410 only

    Available Since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV_SEPT9_i1 D1-25-msfGFP

    Plasmid

    #180333

    Purpose

    mammalian expression of human SEPT9_i1 D1-25 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v1 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT9_i1 D1-25

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV_UdgX-EE-UdgX-NG-nCas9-RBMX

    Plasmid

    #163571

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9-RBMX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-N1_MeCP2(R111G)

    Plasmid

    #110187

    Purpose

    Expression of mouse MeCP2 (R111G) in mammalian cells

    Depositor

    Adrian Bird

    Article

    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

    Insert

    MeCP2_e2 FL [R111G] (mouse cDNA) (Mecp2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Arg 111 Gly

    Promoter

    CMV

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-cT3G-cERP2-TOX2

    Plasmid

    #192920

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of TOX2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    TOX2 (TOX2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV2_hSyn_Aurora_Citrine

    Plasmid

    #98217

    Purpose

    Red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…

    Promoter

    human synapsin

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. H11-mScarletALG8

    Plasmid

    #172440

    Purpose

    Hipp11 targeting vector, expresses mScarlet-mALG8

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Insert

    mScarlet-mALG8
    

    Use

    Mouse Targeting

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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