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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 6281 - 6300 of 16276 results

  1. pB–DualPE

    Plasmid
    #235161
    Purpose
    For plant prime editing including large DNA fragment editing in wheat plants or other monocotyledons
    Depositor
    Zong Yuan
    Publication
    Zhao et al Nat Plants. 2025 Feb;11(2):191-205. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Csy4-P2A-Nls-nCas9-NC-nls-MLV-Nls
    CmYLCV-epegRNA-CaMV poly(A) signal
    Use
    CRISPR
    Mutation
    Detailed in manuscript
    Available since
    Oct. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    235161-plasmid-map-sequence-id-488642

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  2. pAAV uN2C GFP (Ctl)

    Plasmid
    #224355
    Purpose
    Express GFP tagged upsteram ORF of NOTCH2NLC with a control size (12x) of GGC repeats
    Depositor
    Nicolas Charlet-Berguerand
    Publication
    Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.
    Insert
    uN2C
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Codon-optimized for expression in human, so no pu…
    Promoter
    CMV + chimeric intyron (CAG)
    Available since
    Oct. 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    224355-plasmid-map-sequence-id-448162

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  3. pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

    Plasmid
    #218508
    Purpose
    integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase
    Depositor
    Emmanuelle Charpentier
    Publication
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mNeongreen
    Tags
    modified ssrA degradation tag
    Expression
    Bacterial
    Mutation
    deletion of MCS containing Plac_lacZa, introducti…
    Promoter
    P23
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218508-plasmid-map-sequence-id-438746

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  4. pEGFP_IRES_tdTomato

    Plasmid
    #184046
    Purpose
    Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequence
    Depositor
    Angela Pannier
    Publication
    Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Enhanced Green Fluorescent Protein
    tdTomato
    Internal Ribosome Entry Site
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    184046-plasmid-map-sequence-id-363158

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  5. pcDNA3.1-VEGFR3-V5/HIS

    Plasmid
    #205618
    Purpose
    Expression of human VEGFR3 receptor tyrosine kinase in mammalian cells
    Depositor
    Pavel Krejčí
    Publication
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VEGFR3 receptor tyrosine kinase (FLT4 Human)
    Tags
    V5-His
    Expression
    Mammalian
    Mutation
    T28M, S455R- Please see depositor comment
    Promoter
    CMV
    Available since
    Sept. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    205618-plasmid-map-sequence-id-407177

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  8. PB-Neo-TetO-Cdk12_Nterm_FlagHa

    Plasmid
    #127179
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector
    Depositor
    Phil Sharp
    Publication
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Piggybac transposase vector
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mammalian
    Promoter
    TetO Promoter
    Available since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    127179-plasmid-map-sequence-id-247939

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  14. HOXC6 (human) HIS-tag pET

    Plasmid
    #8565
    Depositor
    Corey Largman
    Insert
    HOXC6 (HOXC6 Human)
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    This protein starts at an internal Met initially …
    Available since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  15. pU6-(BsaI)_CBh-UN-Cas9

    Plasmid
    #135011
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9
    Depositor
    Yosef Shaul
    Publication
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Tags
    3xFLAG and 126aa domain from HSV-1 UL12 fused to …
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135011-plasmid-map-sequence-id-267594

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  16. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Plasmid
    #101230
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101230-plasmid-map-sequence-id-198024

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  17. pvPE-V4

    Plasmid
    #240288
    Purpose
    Encodes fourth generation porcine endogenous retrovirus-derived prime editing (pvPE) system into mammalian cells for gene editing.
    Depositor
    Lusheng Huang
    Publication
    Liu et al Trends Biotechnol. 2025 Aug 30:S0167-779
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pvPE-V4
    Use
    CRISPR
    Tags
    SV40 NLS and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    240288-plasmid-map-sequence-id-488805

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  19. mCherry-FKBP-SAC1-HLx8

    Plasmid
    #108136
    Purpose
    Recruitable SAC1 with extended linker
    Depositor
    Gerry Hammond
    Publication
    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
    Expression
    Mammalian
    Mutation
    SACM1L(1-520):[EAAAR]8:SACM1L(521-587)
    Promoter
    CMV
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    108136-plasmid-map-sequence-id-207723

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Showing: 6281 - 6300 of 16276 results