We narrowed to 59,446 results for: cloning

1. pSECRETS-B

   Plasmid

   #196987

   Purpose

   For SECRETS protocol to screen for gRNA activity and specificity: BsaI cassette for gRNA expression or for cloning (x-)gRNA librarys + off-target for SECRETS counter-selection.

   Depositor

   Eric Josephs

   Article

   Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

   Insert

   Spy gRNA cassette
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   pltetO1

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDN0520 (pDEST, TRP1)

   Plasmid

   #210481

   Purpose

   pDEST vector with TRP1 marker for gene expression in S. cerevisiae.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Mol Biol Evol. 2023 Nov 18:msad246. doi:

   Type

   Empty backbone

   Use

   Destination vector for gateway cloning

   Promoter

   ADH1

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJS506

   Plasmid

   #200712

   Purpose

   Promoter region from ATPase PMA1 as Pro5U promoter module for heterologous expression of plasma membrane localized transporters in yeast

   Depositor

   Jens Boch

   Article

   Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi

   Insert

   PMA1 promoter (PMA1 Budding Yeast)
   

   Use

   Synthetic Biology; Modular cloning

   Mutation

   domesticated for MoClo cloning (removal of intern…

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Atg32 [200-341] 1B

   Plasmid

   #190431

   Purpose

   Recombinant expression plasmid of S. cerevisiae Atg32 amino acids 200 - 341 in E. coli.

   Depositor

   Michael Ragusa

   Article

   Xia et al Autophagy. 2018;14(9):1620-1628. doi: 10

   Insert

   Atg32
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Available Since

   July 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. NarCas9_AAV

   Plasmid

   #192145

   Purpose

   Expresses NarCas9?and cloning backbone for sgRNA

   Depositor

   Yongming Wang

   Article

   Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

   Insert

   NarCas9
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDL70

   Plasmid

   #175897

   Purpose

   pENTRY for Gateway LR reaction

   Depositor

   Nemo Peeters

   Article

   Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

   Insert

   ID#13
   

   Use

   Pentry clone

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDL201

   Plasmid

   #175919

   Purpose

   pENTRY for Gateway LR reaction

   Depositor

   Nemo Peeters

   Article

   Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

   Insert

   ID#88
   

   Use

   Pentry clone

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDL108

   Plasmid

   #175913

   Purpose

   pENTRY for Gateway LR reaction

   Depositor

   Nemo Peeters

   Article

   Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

   Insert

   ID#38
   

   Use

   Pentry clone

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDL109

   Plasmid

   #175914

   Purpose

   pENTRY for Gateway LR reaction

   Depositor

   Nemo Peeters

   Article

   Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

   Insert

   ID#40
   

   Use

   Pentry clone

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDL65

    Plasmid

    #175891

    Purpose

    pENTRY for Gateway LR reaction

    Depositor

    Nemo Peeters

    Article

    Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

    Insert

    ID#11
    

    Use

    Pentry clone

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDL69

    Plasmid

    #175905

    Purpose

    pENTRY for Gateway LR reaction

    Depositor

    Nemo Peeters

    Article

    Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

    Insert

    ID#29
    

    Use

    Pentry clone

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDL106

    Plasmid

    #175911

    Purpose

    pENTRY for Gateway LR reaction

    Depositor

    Nemo Peeters

    Article

    Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

    Insert

    ID#36
    

    Use

    Pentry clone

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDL73

    Plasmid

    #175909

    Purpose

    pENTRY for Gateway LR reaction

    Depositor

    Nemo Peeters

    Article

    Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

    Insert

    ID#25
    

    Use

    Pentry clone

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDL110

    Plasmid

    #175915

    Purpose

    pENTRY for Gateway LR reaction

    Depositor

    Nemo Peeters

    Article

    Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

    Insert

    ID#41
    

    Use

    Pentry clone

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDL189

    Plasmid

    #175928

    Purpose

    pENTRY for Gateway LR reaction

    Depositor

    Nemo Peeters

    Article

    Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

    Insert

    ID#73
    

    Use

    Pentry clone

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDL187

    Plasmid

    #175933

    Purpose

    pENTRY for Gateway LR reaction

    Depositor

    Nemo Peeters

    Article

    Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

    Insert

    ID#85
    

    Use

    Pentry clone

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDL226

    Plasmid

    #175929

    Purpose

    pENTRY for Gateway LR reaction

    Depositor

    Nemo Peeters

    Article

    Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

    Insert

    ID#74
    

    Use

    Pentry clone

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDL111

    Plasmid

    #175916

    Purpose

    pENTRY for Gateway LR reaction

    Depositor

    Nemo Peeters

    Article

    Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

    Insert

    ID#42
    

    Use

    Pentry clone

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDL107

    Plasmid

    #175912

    Purpose

    pENTRY for Gateway LR reaction

    Depositor

    Nemo Peeters

    Article

    Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

    Insert

    ID#37
    

    Use

    Pentry clone

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDL215

    Plasmid

    #175925

    Purpose

    pENTRY for Gateway LR reaction

    Depositor

    Nemo Peeters

    Article

    Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

    Insert

    ID#70
    

    Use

    Pentry clone

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge