We narrowed to 11,584 results for: VARS

1. pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A7-gRNA_(CJT89)

   Plasmid

   #226986

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A7)

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA A7 to create OPA1-R194G
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

   Plasmid

   #226992

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 2b to create OPA1-del_exon5
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)

   Plasmid

   #226990

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 1b to create OPA1-del_exon5
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A9-gRNA_(CJT87)

   Plasmid

   #226988

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A9)

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA A9 to create OPA1-R194G
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG8Ha-His-EWSR1-359-549

   Plasmid

   #195868

   Purpose

   Expresses the RRM-RG2-ZnF (359-549) of EWS

   Depositor

   David Libich

   Article

   Selig et al Biopolymers. 2023 May;114(5):e23536. doi

   Insert

   EWS 359-549 (EWSR1 Human)
   

   Tags

   His8-linker-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. macroH2A1.1-F192V-GFP (pc5114)

   Plasmid

   #223437

   Purpose

   Mutation phenylalanine 192 to valine (F192V) in macroH2A1.1-GFP

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

   Insert

   macroH2A1.1-F192V (Macroh2a1 Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti HsATP13A2 P652_E653 del

   Plasmid

   #213700

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP13A2 P652_E653 del

   Depositor

   Veerle Baekelandt

   Article

   Colijn et al Neurogenetics. 2024 Jul 18. doi: 10.1007

   Insert

   human ATP13A2 P652_E653 del (ATP13A2 Human)
   

   Use

   Lentiviral

   Mutation

   WT, D962N

   Promoter

   CMV

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOPINVHH_A8_His

   Plasmid

   #201718

   Purpose

   Expression of nanobody

   Depositor

   Ray Owens

   Article

   Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

   Insert

   VHH_A8 (S lama glama (llama))
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOPINVHH_A10-5_His

   Plasmid

   #201720

   Purpose

   Expression of nanobody

   Depositor

   Ray Owens

   Article

   Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

   Insert

   VHH_A10-5 (S lama glama (llama))
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOPINVHH_B5-5_His

    Plasmid

    #201721

    Purpose

    Expression of nanobody

    Depositor

    Ray Owens

    Article

    Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

    Insert

    VHH B5-5 (S lama glama (llama))
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-enCas12a-A3A-N57G

    Plasmid

    #193651

    Purpose

    Expression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLb-enCas12a-hA3A-N57G, EGFP
    

    Expression

    Mammalian

    Mutation

    dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-N57G

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV6-AN-DDK_S172A_M173A_V195A RAD23A

    Plasmid

    #201576

    Purpose

    Expresses a variant of human RAD23A containing mutations S172A, M173A and V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutations S172A, M173A and V195A

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Stx5L(m55V)-GFP

    Plasmid

    #209845

    Purpose

    Expresses Stx5 M55V mutant fused to GFP in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    STX5 (STX5 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Changed Methionine 55 to Valine, causes loss of S…

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. B-HypaR-SpCas9

    Plasmid

    #126764

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-Hypa2SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A; N692A; M694A; Q695A; H698A; amino acids 10…

    Promoter

    CBh

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ERAP1-rs27895(C)

    Plasmid

    #206142

    Purpose

    Expresses the rs27895-C allele of ERAP1, myc-tagged.

    Depositor

    Dennis Ko

    Article

    Schott et al Cell Genom. 2022 Nov 9;2(11):100207. doi

    Insert

    ERAP1 (ERAP1 Human)
    

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. K365-iLid

    Plasmid

    #188456

    Purpose

    Component of optically-induced motor dimerization system

    Depositor

    Zvonimir Dogic

    Article

    Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

    Insert

    Kinesin 1-365 (Khc Fly)
    

    Tags

    6xHis and iLid

    Expression

    Bacterial

    Mutation

    Kinesin amino acids 1-365

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. K365-micro

    Plasmid

    #188457

    Purpose

    Component of optically-induced motor dimerization system

    Depositor

    Zvonimir Dogic

    Article

    Lemma et al PNAS Nexus. 2023 Apr 12;2(5):pgad130. do

    Insert

    Kinesin 1-365 (Khc Fly)
    

    Tags

    6xHis-MBP-TEV and SspB micro

    Expression

    Bacterial

    Mutation

    Kinesin amino acids 1-365

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGBT-RP8.3

    Plasmid

    #189848

    Purpose

    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 3.

    Depositor

    Stephen Ekker

    Article

    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    mRFP and tagBFP

    Promoter

    beta Actin

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT7-EGFP-C1-HseIF4G1-L640AV646A_AD

    Plasmid

    #148520

    Purpose

    Mammalian Expression of HseIF4G1-L640AV646A

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1-L640AV646A (EIF4G1 Human)
    

    Expression

    Mammalian

    Mutation

    one non silent mutation M432V (natural variant is…

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT7-EGFP-C1-HseIF4G1-LIVVtoAAAA_AD

    Plasmid

    #148521

    Purpose

    Mammalian Expression of HseIF4G1-LIVVtoAAAA

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

    Insert

    HseIF4G1-LIVVtoAAAA (EIF4G1 Human)
    

    Expression

    Mammalian

    Mutation

    one non silent mutation M432V (natural variant is…

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge