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pUC19_HDRT_TRAC_CD19-CAR

Plasmid

#183473

Purpose

Encodes for a homology-directed DNA repair template for the insertion of a second generation CD19-specific CAR into the TRAC gene

Depositor

Dimitrios Wagner

Article

Kath et al Mol Ther Methods Clin Dev. 2022 Apr 12;2

Inserts

TRAC (TRAC Human)
CD19-specific CAR transgene insert
TRAC (TRAC Human)

Use

Unspecified

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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ERK-KTR-mStrawberry reporter

Plasmid

#158686

Purpose

This plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-dTom-nlsdTom (AAV1)

Viral Prep

#135630-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-S5E2-dTom-nlsdTom (#135630). In addition to the viral particles, you will also receive purified pAAV-S5E2-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Tags

dTomato

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only
pMRX-IPU-GFP-MYH9

Plasmid

#168273

Purpose

Expresses MYH9 in mammalian cells

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

MYH9 (MYH9 Human)

Tags

FLAG

Expression

Mammalian

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-SFPQ

Plasmid

#166960

Purpose

Expresses FLAG-tagged SFPQ protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ (SFPQ Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBad-LgBiT-PhoCl1-SmBiT-MBP

Plasmid

#164034

Purpose

luciferase-based PhoCl screening construct

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

LgBiT-PhoCl1-SmBiT-MBP

Tags

His tag, T7 tag, Xpress Tag

Expression

Bacterial

Promoter

araBad promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Mouse Toronto KnockOut (mTKO) CRISPR Library - lentiCRISPRv2

Pooled Library

#159392

Purpose

Mouse lentiviral genome-wide CRISPR library

Depositor

Jason Moffat

Article

Lawson et al Nature. 2020 Oct;586(7827):120-126. doi:

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only
pEGBacmam-NGS Wnt-Fc

Plasmid

#159629

Purpose

Express Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid research

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd7/8 subtype NGS Wnt Fc

Tags

Fc tag and His tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (AAV1)

Viral Prep

#83896-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV-hDlx-GiDREADD-dTomato-Fishell-5 (#83896). In addition to the viral particles, you will also receive purified pAAV-hDlx-GiDREADD-dTomato-Fishell-5 plasmid DNA. hDlx-driven expression of Gi-DREADD and nuclear localized dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Promoter

hDlx

Tags

dTomato (physically separate, not a fusion protein)

Available Since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only
pLenti-mCherry-CDK4KTR

Plasmid

#126680

Purpose

Fluorescent reporter for CDK4/6 activity

Depositor

Hee Won Yang

Article

Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

Insert

RB cterm

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-HA-UFL1

Plasmid

#139860

Purpose

Lentiviral expression of HA-tagged human UFL1

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

UFL1 (UFL1 Human)

Use

Lentiviral

Tags

HA

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLH02

Plasmid

#117262

Purpose

Genome editing for Lactobacillus brevis ATCC367

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

PorfX-LVISKB_1732-30 , sapK-sappR

Use

Other

Available Since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSCM001

Plasmid

#124605

Purpose

Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH

Depositor

David Summers

Article

Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1

Insert

mCherry-pHluorin

Tags

mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…

Expression

Bacterial

Promoter

pDAB

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pV1382

Plasmid

#111436

Purpose

S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-TIWB-yellow pre-eGRASP(p32)

Plasmid

#111582

Purpose

An AAV vector that expresses yellow pre-eGRASP(p32) under the tetO promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

yellow pre-eGRASP(p32)

Use

AAV

Promoter

tetO

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pUNO mouse CD274 + 3'UTR WT full length

Plasmid

#107012

Purpose

Expresses murine PD-L1 with the 3'UTR

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (Cd274 Mouse)

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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U6.3>gRNA.f+e

Plasmid

#99139

Purpose

Empty gRNA scaffold with "Flip" and "Extension" modifications with optimized chicken specific U6 promoter

Depositor

Marianne Bronner

Article

Gandhi et al Dev Biol. 2017 Dec 1;432(1):86-97. doi:

Insert

gRNA

Use

CRISPR

Promoter

chicken U6.3

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-ATP6V0D1

Plasmid

#87931

Purpose

FLAG-tagged overexpression

Depositor

David Sabatini

Article

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

ATP6Vod1 (ATP6V0D1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE2 mCherry-Atl3

Plasmid

#86680

Purpose

Lentivirus to stably express fluorescent protein in mammalian cells

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

ATL3 (ATL3 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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LeGO-mOrange2

Plasmid

#85212

Purpose

Lentiviral vector expressing mOrange2 fluorescent protein driven by SFFV promoter

Depositor

Boris Fehse

Article

Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

Insert

mOrange2

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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