We narrowed to 9,277 results for: Pol;

1. pBactin-Nedd1-mNeonGreen

   Plasmid

   #196864

   Purpose

   Expression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreen

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mNeonGreen (Nedd1 Rat)
   

   Expression

   Mammalian

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS-mTagRFP-TUBA1B

   Plasmid

   #62850

   Purpose

   Donor vector for gene editing at human TUBA1B locus

   Depositor

   Gaudenz Danuser

   Article

   Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

   Insert

   mTagRFP-TUBA1B (TUBA1B Synthetic, Human)
   

   Use

   Yeast-e.coli shuttle vector

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJ4M/TDP-43 S48E

   Plasmid

   #104481

   Purpose

   bacterial expression of TDP-43 S48E

   Depositor

   Nicolas Fawzi

   Article

   Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   MBP-6xHis

   Expression

   Bacterial

   Mutation

   S48E

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBacMam2-DiEx-LIC-C entry vector

   Plasmid

   #111752

   Purpose

   Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with C-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

   Plasmid

   #79887

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Expression

   Bacterial

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1 Flag GFP 4E-BP1 Rheb15

   Plasmid

   #112761

   Purpose

   express GFP-4EBP1-Rheb15

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Flag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)
   

   Use

   Lentiviral

   Tags

   Flag eGFP and Rheb15

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

   Plasmid

   #138524

   Purpose

   Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

   Depositor

   Richard Jenner

   Article

   Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

   Insert

   PRC2-binding G-tract repeat RNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PCRII apoEb

   Plasmid

   #121901

   Purpose

   Plasmid for making zebrafish apoEb in situ probe

   Depositor

   Steven Farber

   Article

   Otis et al Dis Model Mech. 2015 Mar;8(3):295-309. d

   Insert

   Apoeb (apoeb Zebrafish)
   

   Use

   Unspecified

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Frt-V5-hspB2

   Plasmid

   #63103

   Purpose

   Expression of HSPB2 (small HSP) tagged with V5 in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb2 (HSPB2 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX131

    Plasmid

    #167149

    Purpose

    MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    4'-phosphopantetheinyl transferase, npgA
    

    Use

    Synthetic Biology

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. TDP-43_NTD1-80_Y4R

    Plasmid

    #104478

    Purpose

    bacterial expression of TDP-43 NTD Y4R

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y4R

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. psicheck-2-LPPRC-mod

    Plasmid

    #48168

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    LRPPRC leucine rich pentatricopeptide repeat containing (LRPPRC Human)
    

    Use

    Luciferase

    Mutation

    A/T mutation in the ATG of the uORF

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

    Plasmid

    #124278

    Purpose

    Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

    Depositor

    Arthur Laganowsky

    Article

    Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

    Insert

    GIRK2 (Kcnj6 Mouse)
    

    Tags

    eGFP-StreptagII-6xHis

    Expression

    Yeast

    Mutation

    R201A

    Promoter

    AOX1

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST27-GST-ATXN1 FL(92Q)-S776D

    Plasmid

    #21757

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 92 Q(Glutamines) and…

    Promoter

    CMV

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

15. Frt-hspB2

    Plasmid

    #63093

    Purpose

    Expression of HSPB2 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb2 (HSPB2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX028

    Plasmid

    #167151

    Purpose

    MoClo-compatible Level 1 (position 2) vector encoding Neonothopanus nambi hispidin synthase nnHispS under control of 0.4 kb 35S promoter, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    p35s-nnHispS-ocsT
    

    Expression

    Plant

    Promoter

    p35s

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-DN-RFX

    Plasmid

    #52296

    Purpose

    Dominant-negatively inhibits RFX (regulatory factor X) transcription factor. Made of mouse RFX1 DNA binding domain (400-527 aa) and C-terminal myc tag.

    Depositor

    Christopher A Walsh

    Article

    Bae et al Science. 2014 Feb 14;343(6172):764-8. do

    Insert

    Dominant negative RFX
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-2T-TRR-C421-Y2404F

    Plasmid

    #182846

    Purpose

    mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    TRR 2011-2431 (trr Fly)
    

    Expression

    Bacterial

    Mutation

    mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5 GST MetaP2

    Plasmid

    #112750

    Purpose

    express HA-GST-FRB-MetaP2

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    HA-GST-FRB-MetaP2 (METAP2 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQCXIB Flag MFF WT

    Plasmid

    #74384

    Purpose

    Retroviral expression vector containing Flag tagged WT MFF isoform 5

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only