161,472 results

1. B1-INTEGRIN WT

   Plasmid

   #195215

   Purpose

   Mammalian expression of mCherry tagged B1 Integrin. Wild-type.

   Depositor

   Valerie Weaver, David Bryant

   Article

   Paszek et al Cancer Cell. 2005 Sep;8(3):241-54. doi:

   Insert

   ITGB1 (ITGB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDonor14-N7HELIX-KanR

   Plasmid

   #190663

   Purpose

   Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   KanR, R6K origin of replication
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   NA

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMOS028: Mitycam calcium sensor (mitochondrial)

   Plasmid

   #163046

   Purpose

   Mitycam calcium sensor (mitochondrial)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   Mitycam
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-GshF-blasticidin

   Plasmid

   #210313

   Purpose

   Expresses humanized Streptococcus thermophilus GshF in mammalian cells

   Depositor

   Kivanc Birsoy

   Article

   Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

   Insert

   Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
   

   Use

   Retroviral

   Tags

   FLAG peptide

   Expression

   Mutation

   Codon optimized for expression in human cells

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. gGBEv6.3-SpCas9

   Plasmid

   #202629

   Purpose

   vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

   Depositor

   Huawei Tong

   Article

   Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

   Insert

   nSpCas9(D10A)-MPGv6.3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…

   Promoter

   hU6, EF1a, CBh

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. S48I48 ELP

   Plasmid

   #68394

   Purpose

   Elastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residue

   Depositor

   Andrew MacKay

   Article

   Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

   Insert

   S48I48 ELP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSFV3-lacZ

   Plasmid

   #92074

   Purpose

   This is an expression plasmid for production of recombinant genomic SFV RNA encoding beta-galactosidase.

   Depositor

   Henrik Garoff

   Article

   Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

   Insert

   lacZ
   

   Use

   Lacz expression plasmid, for run-off transcriptio…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-EF1a-BbChT (AAV9)

   Viral Prep

   #45186-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from AAV-EF1a-BbChT (#45186). In addition to the viral particles, you will also receive purified AAV-EF1a-BbChT plasmid DNA. Brainbow construct encoding mCherry and mTFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Dawen Cai, Joshua Sanes

   Article

   Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

   Promoter

   EF1a

   Tags

   mCherry, mTFP, or none

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

9. mCherry-TGNP-N-10

   Plasmid

   #55145

   Purpose

   Localization: Golgi Complex, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   TGNP (TGOLN2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-HA-Ubiquitin-K29

    Plasmid

    #22903

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K29 only, all other lysines mutated to arginines

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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