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Showing: 931 - 960 of 8890 results

  5. pSMOX-mNG::LacI-V3

    Plasmid
    #210224
    Purpose
    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.
    Depositor
    Calvin Tiengwe
    Article
    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115
    Insert
    mNeongreen Lac repressor fusion protein
    Use
    Trypanosoma brucei expression
    Tags
    Expression
    Mutation
    Promoter
    Pol II readthrough transcription after integration
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    210224-plasmid-map-sequence-id-415797

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  13. pcpBpaRS_v2

    Plasmid
    #196485
    Purpose
    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40(synthetase), Pol III tRNA promoter (tRNA), C…
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196485-plasmid-map-sequence-id-388866

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  14. pMOD_B2520

    Plasmid
    #91077
    Purpose
    Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    OsU6
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91077-plasmid-map-sequence-id-181905

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  15. pB2-neo-TET

    Plasmid
    #51287
    Purpose
    expresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    sine B2
    Use
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Mutation
    Promoter
    enhancer from 7SL gene
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51287-plasmid-map-sequence-id-78369

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  16. pcpAzpaRS_v2

    Plasmid
    #196483
    Purpose
    To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV40(synthetase), Pol III tRNA promoter (tRNA), C…
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    196483-plasmid-map-sequence-id-388871

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  17. pMOD_B2515

    Plasmid
    #91072
    Purpose
    Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    AtU6
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91072-plasmid-map-sequence-id-181899

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  18. pMOD_B2518

    Plasmid
    #91075
    Purpose
    Module B, Promoter: TaU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    TaU6
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91075-plasmid-map-sequence-id-181902

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  20. pMOD_B2517

    Plasmid
    #91074
    Purpose
    Module B, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    TaU3
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91074-plasmid-map-sequence-id-181901

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  21. pp-CBBC-Fluc

    Plasmid
    #232920
    Purpose
    Allows the Golden Gate (BsmBI) cloning of pegRNA between two Csy4-hairpins in the intron of a CMV-FLUC encoding plasmid (POL II): ipegRNAs must be processed by Csy4 in VLP- producer cells.
    Depositor
    Philippe Mangeot
    Article
    Halegua et al Nat Commun. 2025 Jan 4;16(1):397. doi: 1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232920-plasmid-map-sequence-id-464557

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  25. HIV-1 LTR-gfp

    Plasmid
    #115809
    Purpose
    HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr
    Depositor
    Jeremy Luban
    Article
    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.
    Insert
    gfp and ngfr
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    HIV1 LTR
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    115809-plasmid-map-sequence-id-232377

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  26. pU6-neo-TET

    Plasmid
    #51286
    Purpose
    expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette
    Depositor
    Astrid Roy-Engel
    Article
    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:
    Insert
    U6 (RNU6-1 Human)
    Use
    Tags
    neoTET cassette with a tetrahymena self-splicing …
    Expression
    Mammalian
    Mutation
    Promoter
    U6 upstream enhancer sequence
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    51286-plasmid-map-sequence-id-78368

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  27. pNIFV

    Plasmid
    #111085
    Purpose
    pNIFV is a vector plasmid with mutations in the cis-acting region, to prevent correction of the integrase by recombination when used with pCiPS-NIFV to produce Non-Integrating Foamy Virus vectors
    Depositor
    David Russell
    Article
    Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10
    Insert
    empty backbone/deleted foamy vector, same as pΔΦ with mutations in cis-acting pol region
    Use
    Deleted foamy virus
    Tags
    Expression
    Mutation
    at nt 2334 A changed to T, at nt 2495 A changed t…
    Promoter
    CMV
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    111085-plasmid-map-sequence-id-212298

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  28. pMOD_B2515b

    Plasmid
    #91073
    Purpose
    Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    AtU6
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91073-plasmid-map-sequence-id-181900

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Showing: 931 - 960 of 8890 results