Addgene

We narrowed to 1,957 results for: SUMO

Showing: 931 - 960 of 1957 results

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pJH858

Plasmid

#172176

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH859

Plasmid

#172177

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH750

Plasmid

#172170

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.3f2, deleted sumo

Use

Tags

Expression

Bacterial

Mutation

Evolved PylBCD operon; SUMO tag deleted from PylB…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH751

Plasmid

#172171

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.JM10.1, deleted sumo

Use

Tags

Expression

Bacterial

Mutation

Evolved PylBCD operon; SUMO tag deleted from PylB…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH768v2

Plasmid

#172173

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.3f2]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH769v2

Plasmid

#172174

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1]

Use

Tags

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCDB325

Plasmid

#113669

Purpose

expresses Ulp1_R1 in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R1

Use

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG

Plasmid

#91945

Purpose

Bacterial expression of maize SAE1 and truncated SAE2

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

HA-ZmSAE1
ZmSAE2-FLAG

Use

Tags

FLAG and HA

Expression

Bacterial

Mutation

Truncation of ZmSAE2 to mimic a splice variant

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-SENP1

Plasmid

#17357

Depositor

Edward Yeh

Article

Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

Insert

SENP1 (SENP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 19, 2008

Availability

Academic Institutions and Nonprofits only

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Flag-SENP1m

Plasmid

#17358

Depositor

Edward Yeh

Article

Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

Insert

SENP1 (SENP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

R630L, K631M

Promoter

Available since

March 19, 2008

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2 SENP2

Plasmid

#13382

Depositor

Mary Dasso

Article

Hang et al J Biol Chem. 2002 May 31. 277(22):19961-

Insert

SENP2 (SENP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

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p361_DG

Plasmid

#234683

Purpose

Golden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLJSRSF7

Plasmid

#64693

Purpose

His-MBP-Smt3-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Article

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Use

Tags

CBD, HisTag, MBP, NpuDnaE-N102, and Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pTXG

Plasmid

#52280

Purpose

Bacterial expression of TARGET proteins C-terminally fused to GyrA intein and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Type

Empty backbone

Use

Tags

GyrA-intein-GST

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCARSF63

Plasmid

#64695

Purpose

H6-TRX-Smt3-GB1-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Article

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Use

Tags

CBD, HisTag, NpuDnaE-N102, Smt3, and TRX

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLJSRSF3

Plasmid

#64692

Purpose

His-GST-Smt3-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Article

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Use

Tags

CBD, GST, HisTag, NpuDnaE-N102, and Smt3

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCARSF85

Plasmid

#64694

Purpose

H6-TF-Smt3-GB1-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Article

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Use

Tags

CBD, HisTag, NpuDnaE-N102, Smt3, and TF

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1deltaC(GiVL-1)

Plasmid

#32032

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

H123-N143 replaced by P642-Y645 of GiDicer

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1(1-384)

Plasmid

#32031

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(1-384)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1deltaC(GiVL-2)

Plasmid

#32034

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

N195-M215 replaced by P697-D714 of GiDicer

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1deltaC(E147Q/D151N)

Plasmid

#32024

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

E147Q/D151N

Promoter

T7

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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10xHis-ataxin-3 *SIM-HA

Plasmid

#89983

Purpose

Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

10xHis and HA

Expression

Mammalian

Mutation

mutated aa 162IFVV to 162AFAA

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 *SIM

Plasmid

#89979

Purpose

Expresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutated 162IFVV to 162AFAA

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pET11a-hRanGAP1

Plasmid

#50941

Purpose

expression of full length untagged human RanGAP1 in E.coli

Depositor

Frauke Melchior

Article

Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

Insert

RanGTPase activating protein 1 (RANGAP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 30, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-SENP2

Plasmid

#18047

Depositor

Edward Yeh

Article

Kang et al Mol Cell. 2010 Apr 23. 38(2):191-201.

Insert

SENP2 (SENP2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

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pET28a-HsSENP3

Plasmid

#71467

Purpose

Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 3 (SENP3 Human)

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2 RanGAP

Plasmid

#13378

Depositor

Mary Dasso

Article

Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

Insert

RanGAP (RANGAP1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-SENP2m

Plasmid

#18713

Depositor

Edward Yeh

Article

Kang et al Mol Cell. 2010 Apr 23. 38(2):191-201.

Insert

SENP2 (SENP2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

R577L, K578M

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
pET28a-HsSENP2

Plasmid

#71466

Purpose

Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 2 (SENP2 Human)

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-HsSENP1

Plasmid

#71465

Purpose

Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 1 (SENP1 Human)

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only