We narrowed to 3,810 results for: Pam
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Plasmid#165604PurposeReporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFPDepositorInserthEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
UseSynthetic BiologyExpressionBacterialMutation'CAGCG' PAM replacing the 'CGGCG…PromoterlacAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG198
Plasmid#165605PurposeReporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFPDepositorInserthEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
UseSynthetic BiologyExpressionBacterialMutationSecondary protospacer ('CGGCG' PAM) ins…PromoterlacAvailable SinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG184
Plasmid#165604PurposeReporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFPDepositorInserthEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
UseSynthetic BiologyExpressionBacterialMutation'CAGCG' PAM replacing the 'CGGCG…PromoterlacAvailable SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG198
Plasmid#165605PurposeReporter plasmid for B1H-dependent expression of the HIS3/GFP operon with two hEGFP protospacers: PS1(upstream of promoter with 'CAGCG' PAM)-lac-PS2(downstream with 'CGGCG' PAM)-HIS3 and GFPDepositorInserthEGFP protospacer ('CGGCG' PAM) downstream of the HIS3/GFP promoter
UseSynthetic BiologyExpressionBacterialMutationSecondary protospacer ('CGGCG' PAM) ins…PromoterlacAvailable SinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pxCas9CR4
Plasmid#111656PurposePCas9CR4 with the xCas9 mutations allowing NG PAM sitesDepositorInsertTetR and xCas9
UseSynthetic BiologyTagsssrAExpressionBacterialMutationxCas9 mutationsAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTPH405c
Plasmid#185726PurposeDirected evolution of Cas9 adenine base editors with altered PAM compatibilityDepositorInsertgIII(N)-NpuN-NpuC-gIII(C) single PAM linker
UseCRISPRExpressionBacterialAvailable SinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pxCas9CR4
Plasmid#111656PurposePCas9CR4 with the xCas9 mutations allowing NG PAM sitesDepositorInsertTetR and xCas9
UseSynthetic BiologyTagsssrAExpressionBacterialMutationxCas9 mutationsAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTPH405c
Plasmid#185726PurposeDirected evolution of Cas9 adenine base editors with altered PAM compatibilityDepositorInsertgIII(N)-NpuN-NpuC-gIII(C) single PAM linker
UseCRISPRExpressionBacterialAvailable SinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOLENTE
Plasmid#80683PurposeDEST vector for cell-free in vitro expressionDepositorTypeEmpty backboneUseSynthetic Biology; In vitro transcription and tra…ExpressionMammalianPromoterT7 and SP6Available SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CAAAG
Plasmid#170096PurposedeGFP reporter plasmid with CAAAG PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-CAATG
Plasmid#170097PurposedeGFP reporter plasmid with CAATG PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-GTAAT
Plasmid#170098PurposedeGFP reporter plasmid with GTAAT PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-GTATT
Plasmid#170099PurposedeGFP reporter plasmid with GTATT PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATAAC
Plasmid#170095PurposedeGFP reporter plasmid with ATAAC PAM upstream of p70a promoterDepositorInsertdeGFP
UseSynthetic BiologyExpressionBacterialPromoterp70aAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
WN10150
Plasmid#80443PurposeExpresses dead (908A) AsCpf1DepositorInsertnuclease dead (908A) AsCpf1
TagsNLS-3xHAExpressionMammalianMutationnuclease dead (D908A)Available SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTE4497
Plasmid#80339Purposemammalian expression FnCpf1 nuclease and Fn crRNADepositorInsertsFn crRNA
FnCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMV and human U6Available SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTE4495
Plasmid#80338Purposemammalian expression MbCpf1 nuclease and Mb crRNADepositorInsertsMb crRNA
MbCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianPromoterCMV and human U6Available SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTE4938
Plasmid#107527PurposeExpresses Fn crRNA and mCherry in mammalian cells.DepositorInsertsFn crRNA
mCherry
ExpressionMammalianPromoterCBh and human U6Available SinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTE4999
Plasmid#107555PurposeExpresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.DepositorInserthFnCpf1(dead1)
UseCRISPRTags3xHA and NLSExpressionMammalianMutationD917APromoterCMVAvailable SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTE4560
Plasmid#107526PurposeExpresses As crRNA and mCherry in mammalian cells.DepositorInsertsAs crRNA
mCherry
ExpressionMammalianPromoterCBh and human U6Available SinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only