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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,980 results for: SEC

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Showing: 621 - 640 of 10980 results

  1. pAL942

    Plasmid
    #119721
    Purpose
    precursor slow-folding rbsB with the mutations Alanine 248 Threonine, Alanine 188 Cysteine and Glutamic acid 246 Cysteine
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precusor slow-folding ribose import binding protein, rbsB, mutations A248T, A118C and E246C
    Tags
    none
    Expression
    Bacterial
    Mutation
    Alanine 248 to Threonine, Alanine 188 to Cysteine…
    Promoter
    T7
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119721-plasmid-map-sequence-id-232465

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  3. pAL829

    Plasmid
    #119697
    Purpose
    precursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347C
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    slow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutations Y283D, G289C and V347C
    Promoter
    T5
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119697-plasmid-map-sequence-id-232493

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  4. KTD101(DE3)

    Bacterial Strain
    #138651
    Purpose
    KTD101(DE3) is a trigger factor deficient strain, which may increase protein secretion from Escherichia coli, and is compatible with expression from the T7 promoter
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Bacterial Resistance
    None
    Species
    Escherichia coli
    Available Since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only

  5. pOCC167

    Plasmid
    #118909
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118909-plasmid-map-sequence-id-232266

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  7. pOCC93

    Plasmid
    #118911
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Tags
    eGFP-HIS6
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118911-plasmid-map-sequence-id-232347

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  8. pOCC92

    Plasmid
    #118910
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HBMss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    Tags
    eGFP-HIS6
    Expression
    Insect
    Promoter
    polH
    Available Since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    118910-plasmid-map-sequence-id-234552

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  9. pAL950

    Plasmid
    #119748
    Purpose
    precursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
    Tags
    none
    Expression
    Bacterial
    Mutation
    Internal NdeI site removed and mutations Alanine …
    Promoter
    T7
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119748-plasmid-map-sequence-id-232481

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  11. pGS-BacA-23122

    Plasmid
    #109181
    Purpose
    Plasmid for expression of N-terminally 3xFLAG-tagged proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems
    Depositor
    Monika Golas
    Article
    Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.
    Type
    Empty backbone
    Tags
    3xFLAG
    Expression
    Insect
    Promoter
    minimal HSP70 promoter
    Available Since
    July 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    109181-plasmid-map-sequence-id-232913

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  17. mFzd4CRDL(42-203)-6xHis_pAcGP67a

    Plasmid
    #216379
    Purpose
    Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells
    Depositor
    William Weis
    Article
    Bruguera et al bioRxiv 2024.02.03.578714
    Insert
    Frizzled-4 (Fzd4 Mouse)
    Use
    Baculovirus
    Tags
    6xHis and GP64 signal sequence
    Expression
    Insect
    Promoter
    Polyhedrin
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    216379-plasmid-map-sequence-id-433261

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  18. pAL832

    Plasmid
    #119305
    Purpose
    precusor outer membrane protein A, ompA, truncated after amino acid 176
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
    Tags
    none
    Expression
    Bacterial
    Mutation
    truncated after amino acid 176, mutations Threoni…
    Promoter
    lac
    Available Since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119305-plasmid-map-sequence-id-232460

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  19. pAL800

    Plasmid
    #119306
    Purpose
    precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
    Tags
    none
    Expression
    Bacterial
    Mutation
    amino acids 1 through 171 deleted, and mutatioms …
    Promoter
    lac
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119306-plasmid-map-sequence-id-232254

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  20. pAL928

    Plasmid
    #119853
    Purpose
    precusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427C
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
    Tags
    none
    Expression
    Bacterial
    Mutation
    Cysteine 169 to Serine, Cysteine 179 to Serine, C…
    Promoter
    T7
    Available Since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119853-plasmid-map-sequence-id-232052

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Showing: 621 - 640 of 10980 results