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M-PKD2 (OF2-3)

Plasmid

#21370

Depositor

Gregory Germino

Publication

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type II (PKD2 Human)

Tags

6xMyc

Expression

Mammalian

Mutation

wild-type*

Available since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMV5 HA-TBRII (delta Cyt)

Plasmid

#14051

Depositor

Joan Massague

Publication

Siegel et al Proc Natl Acad Sci U S A. 2003 Jul 8. 10

Insert

TGF beta rec II (delta cyt) (TGFBR2 Human)

Tags

HA (PYDVPDYA)

Expression

Mammalian

Mutation

Dominant negative mutant. A stop codon was introd…

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCAG-Myc-Oct3/4-IP

Plasmid

#13460

Depositor

Shinya Yamanaka

Publication

Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

Insert

Oct3/4 (Pou5f1 Mouse)

Tags

Myc (6x)

Expression

Mammalian

Mutation

None

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA-Flag-H3.3-polyA

Plasmid

#59780

Purpose

Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Publication

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

H3F3B (H3-3B Human)

Expression

Mammalian

Promoter

CMV and T7

Available since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pJRH-1362 scFv entry plasmid

Plasmid

#201912

Purpose

Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

Depositor

Jennifer Doudna

Publication

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain

Expression

Mammalian

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pRSETB/mStayGold(c4)

Plasmid

#212018

Purpose

For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

Depositor

Atsushi Miyawaki

Publication

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Type

Empty backbone

Tags

6xHis, T7 tag, Xpress tag, mStayGold(c4)

Expression

Bacterial

Promoter

T7

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

Plasmid

#134868

Purpose

Expresses EGFP-tagged lysosomal marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Lysosomal-associated membrane protein 1 (LAMP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCS2-UTX-F-MT2

Plasmid

#40619

Purpose

Expression of enzyme-dead UTX in mammalian cells

Depositor

Kai Ge

Publication

Wang et al Proc Natl Acad Sci U S A. 2012 Sep 4.

Insert

UTX (KDM6A Human)

Tags

6xMyc and Flag

Expression

Mammalian

Mutation

changed Histidine 1146 and Glutamic Acid 1148 to …

Promoter

CMV

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pCAG-Sox2-IRES-Neo

Plasmid

#13462

Depositor

Shinya Yamanaka

Publication

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Sox2 (Sox2 Mouse)

Expression

Mammalian

Mutation

None

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRK5-HA-YFP-rictor

Plasmid

#73387

Purpose

Expresses HA and YFP tagged Rictor

Depositor

Jie Chen, Taekjip Ha

Publication

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

RPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)

Tags

HA and YFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG:KikGR

Plasmid

#32608

Depositor

Anna-Katerina Hadjantonakis

Publication

Nowotschin et al BMC Dev Biol. 2009 Sep 9;9:49.

Insert

KikGR

Expression

Mammalian

Promoter

CAG

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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3XFlag pCMV5-TOPO TAZ (S89A)

Plasmid

#24815

Depositor

Jeff Wrana

Publication

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Serine 89 mutated to Alanine

Available since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

12 Articles

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pHJ423

Plasmid

#20681

Depositor

Stephen Shaw

Publication

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Ezrin (EZR Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Thr 567 to Asp

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pmCherry-Sec24D

Plasmid

#32677

Depositor

Henry Lester

Publication

Richards et al J Biol Chem. 2011 Sep 9;286(36):31241-9.

Insert

Sec24D (SEC24D Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

June 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMVTNT-T7-KPNA3

Plasmid

#26679

Depositor

Bryce Paschal

Publication

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 3 (KPNA3 Human)

Tags

T7 (MASMTGGQQMG)

Expression

Mammalian

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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LL - hOCT4i -1

Plasmid

#12198

Depositor

George Daley

Publication

Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

Insert

OCT4 - RNAi (POU5F1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

Sept. 6, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDH-EF1-copGFP-T2A-Puro

Plasmid

#72263

Purpose

Express copGFP and puromycin resistance gene under EF1 promoter

Depositor

Kazuhiro Oka

Insert

copGFP-T2A-Puro

Use

Lentiviral

Expression

Mammalian

Promoter

elongation factor-1

Available since

Jan. 11, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

25 Articles

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p-eGFP-beta 2 CP

Plasmid

#13298

Depositor

John Cooper

Publication

Schafer et al J Cell Biol. 1998 Dec 28. 143(7):1919-30

Insert

capping protein beta 2 subunit (Capzb Mouse)

Tags

EGFP

Expression

Mammalian

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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LYN11-FRB-CFP

Plasmid

#38003

Depositor

Robin Irvine

Publication

Hammond et al Science. 2012 Jun 21.

Inserts

LYN (LYN Human)
MTOR (MTOR )

Tags

ECFP

Expression

Mammalian

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles
pLCA.66/2272

Plasmid

#22733

Depositor

Mark Magnuson

Publication

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox66-pU(delta)TK-EM7Neo-Lox2272

Use

Cre/Lox; Bac recombineering

Mutation

This vector was designed for assembling gene targ…

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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