We narrowed to 10,582 results for: plasmids 101

1. pMCSG53_Nsp1

   Plasmid

   #167256

   Purpose

   Bacterial expression of Nsp1 residues 13-127

   Depositor

   Alexei Savchenko

   Article

   Semper et al iScience. 2021 Jan 22;24(1):101903. doi:

   Insert

   Nsp1 (residues 13-127) (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His6-TEV site

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available Since

   April 27, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pZBTB11-donor

   Plasmid

   #112348

   Purpose

   CRISPR donor plasmid to tag human transcription factor ZBTB11 with GFP

   Depositor

   Kevin White

   Insert

   ZBTB11 homology arms flanking EGFP-IRES-Neo cassette (ZBTB11 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:3:101651181:A>T

   Promoter

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRR-PR-5Z

   Plasmid

   #23057

   Depositor

   Charles Miller

   Article

   Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

   Insert

   PR (PGR Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE30del

   Plasmid

   #106902

   Purpose

   pQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. punc-119c

   Plasmid

   #21595

   Depositor

   Al Fisher

   Article

   Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

   Insert

   unc-119 (unc-119 Nematode)
   

   Use

   Adds unc-119 marker to amp resistant plasmids

   Tags

   Expression

   Worm

   Mutation

   N/A

   Promoter

   Available Since

   Aug. 26, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. punc-119cR

   Plasmid

   #21596

   Depositor

   Al Fisher

   Article

   Ferguson et al Plasmid. 2009 Sep;62(2):140-5. doi: 10.1

   Insert

   unc-119:mCherry (unc-119 Nematode)
   

   Use

   Adds unc-119 marker to amp resistant plasmids

   Tags

   mCherry

   Expression

   Worm

   Mutation

   N/A

   Promoter

   Available Since

   Aug. 26, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. Integrin-Alpha2-N-18

   Plasmid

   #58192

   Purpose

   Localization: Integrin/Focal Adhesions, Excitation: N/A, Emission: N/A

   Depositor

   Michael Davidson

   Insert

   Integrin-Alpha2 (ITGA2 Human)
   

   Use

   Tags

   SNAP tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. CACNA2D1-TwinStrepII

   Plasmid

   #83192

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Insert

   CACNA2D1 (Cacna2d1 Rat)
   

   Use

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Casp2 C303A-Flag

   Plasmid

   #11812

   Depositor

   Guy Salvesen

   Insert

   Caspase-2 catalytic mutant (CASP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   C303A

   Promoter

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pET23b-Casp2-Delta Card

    Plasmid

    #11810

    Depositor

    Guy Salvesen

    Insert

    Caspase-2 Delta Card (CASP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Delta Card

    Promoter

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pCMV6-XL4 FLAG-CBLN2-Fc

    Plasmid

    #115767

    Purpose

    Expression of the extracellular domain of CBLN2 with N-terminal FLAG tag and C-terminal hIgG tag.

    Depositor

    Davide Comoletti

    Insert

    CBLN2 (CBLN2 Human)
    

    Use

    Tags

    FLAG and hIgG/Fc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKG187

    Plasmid

    #63077

    Purpose

    Retroviral expression of EGFP-TEV-S-tag FAAP24 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    FAAP24, IMAGE clone 3659284 (FAAP24 Human)
    

    Use

    Retroviral

    Tags

    EGFP and TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIVTR(T7A)-ATOH1 (SA Substitutions)

    Plasmid

    #172309

    Purpose

    In vitro transcription of ATOH1 (Ser phosphosites substituted by Ala)

    Depositor

    Patrik Verstreken

    Insert

    ATOH1 (ATOH1 Human)
    

    Use

    Other

    Tags

    Expression

    Mutation

    S331A, S342A

    Promoter

    T7 promoter A-initiating

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLNCX1 Myr AKT2

    Plasmid

    #27294

    Depositor

    Morris Birnbaum

    Insert

    protein kinase B beta (AKT2 Human)
    

    Use

    Retroviral

    Tags

    HA and myristolation sequence

    Expression

    Mutation

    PH domain deleted (aa1-107)

    Promoter

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

15. IDG_KCNG3_OE_1

    Plasmid

    #161644

    Purpose

    Overexpresses KCNG3

    Depositor

    Mike McManus

    Insert

    KCNG3 (KCNG3 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. IDG_FAM26E_OE_1

    Plasmid

    #161615

    Purpose

    Overexpresses FAM26E

    Depositor

    Mike McManus

    Insert

    FAM26E (FAM26E Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

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17. G3BP1-Grx1-roGFP2

    Plasmid

    #236548

    Purpose

    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in stress granules.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    G3BP1-Grx1-roGFP2 (G3BP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV-FoxP3-R337Q-IRES-Thy1.1

    Plasmid

    #229617

    Purpose

    Mammalian expression of full length mouse FoxP3 (R337Q)

    Depositor

    Sun Hur

    Insert

    full length FoxP3, carrying single point mutation R337Q (Foxp3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R337Q

    Promoter

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-FoxP3-A372S-IRES-Thy1.1

    Plasmid

    #229618

    Purpose

    Mammalian expression of full length mouse FoxP3 (A372S)

    Depositor

    Sun Hur

    Insert

    full length FoxP3, carrying single point mutation A372S (Foxp3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A372S

    Promoter

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1-TetOn-3XFLAG-firefly-beta-globin-control-AAVS1

    Plasmid

    #184395

    Purpose

    Donor plasmid for stable integration of firefly luciferase control reporter at AAVS1

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Firefly luciferase beta-globin
    

    Use

    CRISPR; Donor plasmid for hdr

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On 3G

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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