We narrowed to 9,863 results for: plasmids 101

1. AAVS1-TetOn-3XFLAG-renilla-beta-globin-PTC39-AAVS1

   Plasmid

   #184398

   Purpose

   Donor plasmid for stable integration of Renilla luciferase NMD(+) PTC39 reporter at AAVS1

   Depositor

   Robert Bradley

   Article

   Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

   Insert

   Renilla luciferase beta-globin-PTC39
   

   Use

   CRISPR; Donor plasmid for hdr

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   PTC in beta-globin at amino acid position 39

   Promoter

   Tet-On 3G

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. MIP 247 CoMiP 4in1 with shRNA p53

   Plasmid

   #63726

   Purpose

   To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

   Depositor

   Joseph Wu, Mark Kay

   Article

   Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

   Insert

   Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC, TP53 Human)
   

   Use

   Tags

   IRES-dtomato

   Expression

   Mammalian

   Mutation

   codon-optimised

   Promoter

   SFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJB94

   Plasmid

   #208752

   Purpose

   Theophylline-based control of repA on a C. difficile plasmid for use in allelic exchange

   Depositor

   Joseph Sorg

   Article

   Brehm et al Anaerobe. 2024 Apr 29;88:102858. doi: 10

   Insert

   sacB (sacB )
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLKO-Tet-puro-hRAF1-shRNA-1

   Plasmid

   #185371

   Purpose

   For tetracycline-inducible mammalian expression of shRNA: CATGAGTATTTAGAGGAAGTA that targets human RAF1

   Depositor

   Ayaz Najafov

   Insert

   RAF1 (RAF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-Fon/ConN2cG

   Plasmid

   #234709

   Purpose

   pAAV transfer plasmid with Ef1a promoter driving Cre- and Flp-dependent expression of G protein for rabies N2c delta G

   Depositor

   David Ng

   Article

   Mendelsohn et al bioRxiv [Preprint]. 2024 Sep 18:2024.08.

   Insert

   Fon Con N2cG
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. BMP2K

   Plasmid

   #73251

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   BMP2KA (BMP2K Human)
   

   Use

   Tags

   His6-Zb-TEV

   Expression

   Bacterial

   Mutation

   pfam00069:Pkinase 52-311

   Promoter

   T7

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pCDZ2FE_Champ1_1-812

   Plasmid

   #105675

   Purpose

   Expresses Flag-tagged full-length human Champ1 protein

   Depositor

   Thanos Halazonetis

   Insert

   Champ1 (CHAMP1 Human)
   

   Use

   Tags

   2X Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGLIS3-donor

   Plasmid

   #113812

   Purpose

   CRISPR donor plasmid to tag human transcription factor GLIS3 with GFP

   Depositor

   Kevin White

   Insert

   GLIS3 homology arms flanking EGFP-IRES-Neo cassette (GLIS3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   rs10118031, rs10973702, rs912257

   Promoter

   Available since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV-SARS-CoV-2 SL1 alone-Luciferase

   Plasmid

   #191482

   Purpose

   Luciferase reporter for SARS-CoV-2 5' UTR SL1 alone

   Depositor

   Hao Wu

   Article

   Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

   Insert

   SARS-CoV-2 5' UTR SL1 alone
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mutation

   Contains only the stem-loop1 region of SARS-CoV-2…

   Promoter

   Available since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV-SARS-CoV-2 5’UTR ΔSL1-Luciferase

    Plasmid

    #191481

    Purpose

    Luciferase reporter for SARS-CoV-2 5' UTR ΔSL1

    Depositor

    Hao Wu

    Article

    Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

    Insert

    SARS-CoV-2 5' UTR ΔSL1
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mutation

    Missing the stem-loop 1 region of SARS-CoV-2 5&#x…

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pANG03

    Plasmid

    #178085

    Purpose

    Tetracycline resistant backbone for in vivo Gateway reaction. Plasmid contains gateway cassette with restriction sites to add promoters or C and N Terminal tags. Compatible with pANG02.

    Depositor

    Sören Abel

    Article

    Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    NA

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pANG01

    Plasmid

    #178083

    Purpose

    Carbenicillin resistant backbone for in vivo Gateway reaction. Plasmid contains gateway cassette with restriction sites to add promoters or C and N Terminal tags. Compatible with pANG02.

    Depositor

    Sören Abel

    Article

    Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    NA

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCW-SPI1

    Plasmid

    #162824

    Purpose

    Doxycycline-inducible overexpression of human SPI1

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet ON

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSPORT1[NheI/glGFP/bglobin3'-UTR/Ins2]

    Plasmid

    #74101

    Purpose

    vector bearing an NheI restriction site for the two-step cloning method in Wang and Szaro, Dev Biol, 2015

    Depositor

    Ben Szaro

    Article

    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

    Inserts

    Green Lantern Green Fluorescent Protein
    Rabbit beta 1-globin gene 3'UTR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. T4 Lysozyme I3A

    Plasmid

    #18200

    Depositor

    Brian Matthews

    Insert

    T4 Lysozyme (e Bacteriophage T4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I3A

    Promoter

    Available since

    Aug. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

16. T4 Lysozyme I3P

    Plasmid

    #18211

    Depositor

    Brian Matthews

    Insert

    T4 Lysozyme (e Bacteriophage T4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I3P

    Promoter

    Available since

    Aug. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

17. T4 Lysozyme I3Y

    Plasmid

    #18215

    Depositor

    Brian Matthews

    Insert

    T4 Lysozyme (e Bacteriophage T4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I3Y

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

18. T4 Lysozyme I3C

    Plasmid

    #18201

    Depositor

    Brian Matthews

    Insert

    T4 Lysozyme (e Bacteriophage T4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I3C

    Promoter

    Available since

    Aug. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

19. T4 Lysozyme I3E

    Plasmid

    #18205

    Depositor

    Brian Matthews

    Insert

    T4 Lysozyme (e Bacteriophage T4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I3E

    Promoter

    Available since

    Aug. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

20. T4 Lysozyme I3F

    Plasmid

    #18206

    Depositor

    Brian Matthews

    Insert

    T4 Lysozyme (e Bacteriophage T4)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I3F

    Promoter

    Available since

    Aug. 26, 2008

    Availability

    Academic Institutions and Nonprofits only