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p2xFlag CMV2-YAP1

Plasmid

#19044

Depositor

Marius Sudol

Publication

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine, and Proline 2…

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pUB39

Plasmid

#26300

Depositor

George Cross

Insert

VSG117 (Lister 427-4) cDNA

Use

Trypanosome inducible expression vector

Available since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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p3E-mTagBFP

Plasmid

#75175

Purpose

TagBFP (blue) fluorophore with stop codon for C-terminal fusions

Depositor

Nicholas Cole

Publication

Don et al Zebrafish. 2016 Sep 15.

Insert

TagBFP

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mouse CENP-B-EGFP

Plasmid

#107264

Purpose

mouse CENP-B-EGFP in pIVT vector for in vitro transcription

Depositor

Michael Lampson

Publication

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

CENP-B-EGFP (Cenpb Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

T7

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p15aC-1D

Plasmid

#107863

Purpose

Bacterial expression of PIS

Depositor

Sanford Simon

Publication

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

PIS

Tags

myc

Expression

Bacterial

Promoter

proD

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR TERRA

Plasmid

#111383

Purpose

TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

Depositor

Klaus Holzmann

Publication

Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

Insert

TERRA

Use

Gateway entry cloning

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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SpyTag-RBD

Plasmid

#159999

Purpose

Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Publication

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)

Expression

Mammalian

Promoter

CMV

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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HA-VHL W117R-pBabe-puro

Plasmid

#19239

Depositor

William Kaelin

Publication

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tryptophan 117 to Arginin

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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meffRed chromoprotein

Plasmid

#117836

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses meffRed chromoprotein in E. coli

Depositor

Anthony Forster

Publication

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, meffRed

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pE1c

Plasmid

#17461

Purpose

modified pENTR vector for C-terminal STREP tag fusion with your gene of interest

Depositor

Giovanna Benvenuto

Publication

Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

Type

Empty backbone

Use

Modified gateway entry vector

Tags

STREP

Expression

Bacterial, Insect, Mammalia…

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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pME-QFGal4-SV40pA

Plasmid

#155118

Purpose

Middle entry vector containing QFGal4

Depositor

Bret Pearson

Publication

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QFGal4

Use

Gateway middle entry vector

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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PNCA-luciferase

Plasmid

#67793

Purpose

Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Publication

Wang et al J Virol. 2015 Mar;89(5):2813-9. doi: 10.

Insert

MMLV-LTR-firefly luciferase

Expression

Mammalian

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pYPQ166-SpRY

Plasmid

#161520

Purpose

Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter

Depositor

Yiping Qi

Publication

Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

Insert

zSpRYCas9

Use

CRISPR; Gateway compatible zsprycas9 entry clone

Tags

3X FLAG, NLS and NLS

Expression

Plant

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MXS_CreERT2

Plasmid

#62427

Purpose

MXS_chaining vector with CreERT2

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tamoxifen inducible Cre recombinase

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pGAGmEos2-N1

Plasmid

#46372

Depositor

Suliana Manley

Publication

Gunzenhauser et al Nano Lett. 2012 Sep 12;12(9):4705-10. do

Insert

Gag

Tags

mEos2

Expression

Mammalian

Mutation

Rev independentHXB2 Gag sequence

Promoter

CMV

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR R4-mCherry-R3

Plasmid

#32311

Depositor

Steve Stowers

Publication

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

mCherry

Use

Gateway multisite entry clone

Available since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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VCV

Plasmid

#27788

Depositor

Steven Vogel

Publication

Koushik et al PLoS One. 2009 Nov 25;4(11):e8031. doi:

Insert

Venus-Cerulean-Venus

Use

Stoichiometry standard

Expression

Mammalian

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles
pIVT-R2Tg-WT

Plasmid

#238105

Purpose

For IVT of wildtype R2Tg editor, codon optimized for mammalian expression.

Depositor

Feng Zhang

Publication

Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

Insert

Wildtype R2Tg, mammalian codon optimized

Use

For ivt

Available since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_Flag_TP53_R248W

Plasmid

#183114

Purpose

Expresses flag-tagged p53 with R248W mutation in mammalian cells

Depositor

William Kaelin

Publication

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed arginine 248 to tryptophan

Promoter

EF1a

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ262B-ABE8e

Plasmid

#161524

Purpose

Gateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editing

Depositor

Yiping Qi

Publication

Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

Insert

TadA8e-zSpRYCas9(D10A)

Use

CRISPR; Gateway compatible tada8e-zsprycas9(d10a)…

Expression

Plant

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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