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pGL3-MTS-ccdb-DddA(G1333C)-UGI-Puro

Plasmid

#173228

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ccdb-DddA(G1333N)-UGI-Puro

Plasmid

#173229

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHD-3xP3-dsRed-DattP

Plasmid

#133559

Purpose

Backbone vector for CRISPR-HDR insertion of SPARC-Variant constructs

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLPE1

Plasmid

#209981

Purpose

Contains Level 0 Part: E. coli shuttle part (pMB1_ampR_B0017) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

E. coli shuttle part (pMB1_ampR_B0017)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPE2

Plasmid

#209982

Purpose

Contains Level 0 Part: E. coli shuttle part (ampR_B0017) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

E. coli shuttle part (ampR_B0017)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPE3

Plasmid

#209985

Purpose

Contains Level 0* Part: E. coli shuttle part (pMB1_cat) for the construction of Level 2 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

E. coli shuttle part (pMB1_cat)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLPP5

Plasmid

#209967

Purpose

Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P_sppQ)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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RVD-AA5

Plasmid

#173240

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Insert

NI-NI dimer RVD at Position5

Use

Unspecified

Mutation

no

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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RVD-AA9

Plasmid

#173244

Purpose

One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

Depositor

Bin Shen

Article

Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

Insert

NI-NI dimer RVD at Position9

Use

Unspecified

Mutation

no

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_AC-CEN_ARS_HIS

Plasmid

#202129

Purpose

Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

CEN_ARS_HIS

Use

Synthetic Biology

Mutation

ARS sequence mutated to remove sapI site for dome…

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C2-TetR-KRf-TetR

Plasmid

#216270

Purpose

Construct 3: TetR - Lipid binding domain KRφ - TetR

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

TetR - Lipid binding motif (KRf) - TetR

Tags

EGFP

Expression

Mammalian

Available Since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-GW

Plasmid

#194634

Purpose

Gateway destination vector for mammalian expression of Virotrap bait constructs

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

p55 GAG

Tags

PQS1

Expression

Mammalian

Promoter

SRalpha

Available Since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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UMG-SP-1_pET29b

Plasmid

#215433

Purpose

Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-1

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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UMG-SP-2_pET29b

Plasmid

#215434

Purpose

Expression construct for UMG-SP-2 gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

UMG-SP-2

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pconst #5-RFP

Plasmid

#81079

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23110

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-Csy4-Cas9n-EGFP-L2

Plasmid

#141046

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Csy4-2A-Cas9n-2A-EGFP

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-RNF169

Plasmid

#99392

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF169

Depositor

Michael Huen

Article

Chen et al J Biol Chem. 2012 Aug 10;287(33):27715-2

Insert

RNF169 (RNF169 Human)

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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NylB_pET29b

Plasmid

#215414

Purpose

Expression construct for NylB gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

NylB

Expression

Bacterial

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-PKCg-C1

Plasmid

#112266

Purpose

ITRACKgamma Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase c gamma (Prkcg Mouse)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pL0_EF-PtBle

Plasmid

#202180

Purpose

Level 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

ShBle expression cassette

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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