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416Gal-FUS-R514G-YFP

Plasmid

#29616

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

R514G

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pHH0103_ITSN1-2/5

Plasmid

#91385

Purpose

Protein expression and purification of human SH3 domain construct ITSN1-2/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN1-2/5 (ITSN1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_LASP1-1/1-SV1/2

Plasmid

#91399

Purpose

Protein expression and purification of human SH3 domain construct LASP1-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LASP1-1/1-SV1/2 (LASP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
DLG3_PDZ_3

Plasmid

#103948

Purpose

Protein expression and purification of NE-DLG PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

DLG3 (DLG3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28A-p53 (1-73) (P to A)

Plasmid

#62069

Purpose

Expression of human p53 (1-73) (all P to A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; all Prolines changed …

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pHH0103_GRB2-2/2

Plasmid

#91410

Purpose

Protein expression and purification of human SH3 domain construct GRB2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GRB2-2/2 (GRB2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_GRAP2-2/2

Plasmid

#91368

Purpose

Protein expression and purification of human SH3 domain construct GRAP2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

GRAP2-2/2 (GRAP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_EPS8L2-1/1

Plasmid

#91526

Purpose

Protein expression and purification of human SH3 domain construct EPS8L2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

EPS8L2-1/1 (EPS8L2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pfast bac cry1myc

Plasmid

#51892

Purpose

Backbone plasmid is pfastBacHTa from invitrogen

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

cry1 (Cry1 Mouse)

Tags

3x Flag and myc

Promoter

polyhedrin promoter

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
PTPN13_PDZ_3

Plasmid

#103900

Purpose

Protein expression and purification of PTPN13 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MAGI1 (MAGI1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_SRC-1/1-IS1/2

Plasmid

#91349

Purpose

Protein expression and purification of human SH3 domain construct SRC-1/1-IS1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SRC-1/1-IS1/2 (SRC Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_CRK-2/2-SV2/2

Plasmid

#91343

Purpose

Protein expression and purification of human SH3 domain construct CRK-2/2-SV2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CRK-2/2-SV2/2 (CRK Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
MAGI1_PDZ_2

Plasmid

#103899

Purpose

Protein expression and purification of MAGI1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

MAGI1 (MAGI1 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
USPQ-HTT-TALEN1

Plasmid

#92243

Purpose

TALEN targeting upstream of HTT gene (KKR), forms obligate heterodimer with USPQ-HTT-TALEN2

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

TALEN

Use

TALEN

Promoter

CMV

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
USPQ-HTT-TALEN2

Plasmid

#92244

Purpose

TALEN targeting upstream of HTT gene (ELD), forms obligate heterodimer with USPQ-HTT-TALEN1

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

TALEN

Use

TALEN

Promoter

CMV

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pfast bac Rbx-HA

Plasmid

#51893

Purpose

Backbone plasmid is pfastBacHTa from invitrogen

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

Rbx1 (RBX1 Human)

Tags

HA

Promoter

polyhedrin promoter

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pTE3320

Plasmid

#107529

Purpose

Expresses human codon optimized LbCpf1(RVR mutant) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hLbCpf1(RVR)

Tags

3xHA and NLS

Expression

Mammalian

Mutation

G532R, K538V, Y542R

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_ASAP1-1/1

Plasmid

#91501

Purpose

Protein expression and purification of human SH3 domain construct ASAP1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ASAP1-1/1 (ASAP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
416Gal-FUS-R518K-YFP

Plasmid

#29621

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

R518K

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
416Gal-FUS-R524T-YFP

Plasmid

#29627

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

R524T

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only