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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,359 results for: cas9

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Showing: 6401 - 6420 of 14359 results

  1. pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP

    Plasmid
    #117140
    Purpose
    Express gRNA vs Dmap1
    Depositor
    Kosuke Yusa
    Insert
    gDmap1 v1 (Dmap1 Synthetic)
    Use
    CRISPR and Lentiviral
    Mutation
    Deleted BbsI site within WPRE, modified BFP
    Promoter
    human U6 promoter, mouse PGK promoter
    Available since
    April 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    117140-plasmid-map-sequence-id-233768

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  2. pAf-CRISPR-yA

    Plasmid
    #191015
    Purpose
    CRISPR vector based on Aspergillus flavus U6 promoter and terminator for targeting the yA gene, which contains AMA1(the HindIII-PstI fragment) and ptrA selection marker.
    Depositor
    Perng-Kuang Chang
    Publication
    Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    yA
    Use
    CRISPR
    Promoter
    Aspergillus flavus U6
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    191015-plasmid-map-sequence-id-516753

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  4. BB44_pmc.DonorR5.TS

    Plasmid
    #199223
    Purpose
    Donor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locus
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Expression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
    Use
    Gene targeting donor plasmid
    Expression
    Mammalian
    Promoter
    Human PGK1 gene promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199223-plasmid-map-sequence-id-391672

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  12. pX330-COSMC-KO

    Plasmid
    #80008
    Purpose
    gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.
    Depositor
    Sriram Neelamegham
    Publication
    Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    COSMC (C1GALT1C1 Human)
    Use
    CRISPR
    Available since
    July 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    80008-plasmid-map-sequence-id-153264

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  18. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid
    #174136
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174136-plasmid-map-sequence-id-341914

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  19. PX458_ATF4_2

    Plasmid
    #72352
    Purpose
    Encodes gRNA for 3' target of human ATF4 along with Cas9 with 2A GFP
    Depositor
    Eric Mendenhall, Richard M. Myers
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ATF4 (ATF4 Human)
    Use
    CRISPR
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72352-plasmid-map-sequence-id-134607

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  20. pDM030

    Plasmid
    #216810
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and ble selectable marker.
    Depositor
    Ken Sawin
    Publication
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216810-plasmid-map-sequence-id-430137

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Showing: 6401 - 6420 of 14359 results