We narrowed to 150,751 results for: ins

1. pFastBac HT JS-Munc18b

   Plasmid

   #135554

   Purpose

   Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   Munc18b (Stxbp2 Rat)
   

   Tags

   6xHis tag and TEV site

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. VRK3_HUMAN_D0

   Plasmid

   #79681

   Purpose

   This plasmid encodes the kinase domain of VRK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   VRK3 (VRK3 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pHBS953 TARDBP-Exon2-ko-sgRNA2-SpCas9

   Plasmid

   #107858

   Purpose

   To knock-out TDP43

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   gRNA against TARDBP (Exon 2) (TARDBP Human)
   

   Expression

   Mammalian

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

   Plasmid

   #115139

   Purpose

   CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS

   Depositor

   Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized AcrVA3.1 anti-CRISPR protein
   

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. GST-rhodopsin-CTS

   Plasmid

   #86071

   Purpose

   A bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Rhodopsin (RHO Bovine)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains the region from 309-348 of Rhodopsin

   Promoter

   tac

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-p53 (Mouse 1-87)

   Plasmid

   #62061

   Purpose

   Expression of Mouse p53 (residues 1-87) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

   Insert

   p53 (Trp53 Mouse)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-87

   Promoter

   T7

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

7. PHKG2_HUMAN_D0

   Plasmid

   #79736

   Purpose

   This plasmid encodes the kinase domain of PHKG2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   PHKG2 (PHKG2 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. VRK2_HUMAN_D0

   Plasmid

   #79737

   Purpose

   This plasmid encodes the kinase domain of VRK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   VRK2 (VRK2 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pAcrF1

   Plasmid

   #89233

   Purpose

   Plasmid contains the gene for anti-CRISPR protein AcrF1 (gene 35 from bacteriophage JBD30), with N-terminal 6his tag and TEV protease cleavage site

   Depositor

   Blake Wiedenheft

   Article

   Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

   Insert

   AcrF1
   

   Tags

   6xHis-TEV and FLAG

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Gcm2TEASY

    Plasmid

    #41031

    Depositor

    Nancy Manley

    Article

    Gordon et al Mech Dev. 2001 May;103(1-2):141-3.

    Insert

    Gcm2 in situ probe (Gcm2 Mouse)
    

    Use

    In situ probe

    Mutation

    contains nucleotides 584-1510 of GenBank accessio…

    Promoter

    T7 sense; SP6 antisense

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pX4

    Plasmid

    #183903

    Purpose

    donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    GFP
    

    Expression

    Insect

    Promoter

    Aedes aegypti PUb

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX3

    Plasmid

    #183904

    Purpose

    donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    GFP
    

    Expression

    Insect

    Promoter

    Aedes aegypti Polyubiquitin

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABEpuro-ARAF_S214C

    Plasmid

    #51077

    Purpose

    mammalian expression of ARAF S214C

    Depositor

    Matthew Meyerson

    Article

    Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

    Insert

    ARAF (ARAF Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    S214C

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX SH2A-SH2

    Plasmid

    #46481

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SH2A (SH2D4A Human)
    

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 338-445

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. PF3D7_0606800-bio

    Plasmid

    #47790

    Purpose

    Expresses enzymatically monobiotinylated full-length PF3D7_0606800 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised PF3D7_0606800
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX Slap2-SH2

    Plasmid

    #46501

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Slap2 (SLA2 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 84-197

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX Lnk-SH2

    Plasmid

    #46451

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Lnk (Sh2b3 Mouse)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 325-466

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

    Plasmid

    #115140

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

    Depositor

    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA3 anti-CRISPR protein
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

    Plasmid

    #115138

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

    Depositor

    Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized AcrVA2 anti-CRISPR protein
    

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVENUS-N1-FNTA

    Plasmid

    #86859

    Purpose

    To express FNTA with a Venus tag at C terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    FNTA (FNTA Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    The sequence of the insert is isoform 2, which di…

    Promoter

    CMV

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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