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160,228 results

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SJZ9-amdS

Plasmid

#228261

Purpose

Template plasmid for yeast gene knockout, allows the use of acetamide as sole nitrogen source

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

amdS

Use

Tags

Expression

Yeast

Mutation

Promoter

ENO2 S.c.

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET19-TdTwt

Plasmid

#207466

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

TdTwt (Dntt Mouse)

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti.PGK.blast-Renilla_Luciferase

Plasmid

#74444

Purpose

Control lentiviral vector expressing Renilla luciferase

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Renilla Luciferase

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-p65

Plasmid

#111190

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

RELA (RELA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pFH2.22_mCherry-MS2x8

Plasmid

#205537

Purpose

Express mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.0 FLAG TRPV4

Plasmid

#45751

Depositor

Robert Lefkowitz

Article

Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

Insert

TRPV4 (Trpv4 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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p0521s

Plasmid

#62862

Purpose

Episome vector for Phaeodactylum tricornutum (pCC1BAC-based)

Depositor

Hamilton Smith

Article

Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

Type

Empty backbone

Use

Synthetic Biology; Episomal vector for phaeodacty…

Tags

Expression

Mutation

Promoter

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pFBDM

Plasmid

#110738

Purpose

Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin and p10 promoters

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX307-TMPRSS2-blast

Plasmid

#158458

Purpose

Constitutive lentiviral expression of human TMPRSS2.

Depositor

Sho Iketani, Alejandro Chavez

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCDH-METTL14-WT

Plasmid

#141112

Purpose

Overexpression of WT METTL14 in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

HMETTL14 (METTL14 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TLR3-CFP

Plasmid

#13641

Depositor

Doug Golenbock

Insert

Toll-Like Receptor 3 (TLR3 Human)

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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pX330S-2

Plasmid

#58778

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pOlyA(E69A)

Plasmid

#132392

Purpose

Mutant version of OlyA that senses sphingomyelin without or with cholesterol

Depositor

Arun Radhakrishnan

Article

Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

Insert

Ostreolysin A point mutant (E69A)

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: C62S, E69A, C94S, S151C

Promoter

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-PP7-2xmCherry-CAAX

Plasmid

#74925

Purpose

Expresses PP7-2xmCherry-CAAX

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

PP7-2xmCherry-CAAX

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMX229

Plasmid

#23002

Depositor

Linda Wordeman

Article

Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

Insert

Kif18A (KIF18A Human)

Use

Tags

6His and EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-Cyclin D1

Plasmid

#19927

Depositor

Yue Xiong

Article

Watanabe et al Proc Natl Acad Sci U S A. 1998 Feb 17. 9

Insert

Cyclin D1 (CCND1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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pLVX-SRRM2-mCherry

Plasmid

#174089

Purpose

Inducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on system

Depositor

Wesley Sundquist, Katie Ullman

Article

Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

Insert

SRRM2 (SRRM2 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

TREtight

Available since

Sept. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-Ftractin-mCherry

Plasmid

#85131

Purpose

lentiviral expression of an F-actin live-cell marker (mCherry)

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

EF1a

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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3xERRE/ERE-luciferase

Plasmid

#37852

Depositor

Rebecca Riggins

Article

Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

Insert

3 copies of ERRE/ERE upstream of luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

SV40

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pBS_nanorod_105b

Plasmid

#120347

Purpose

phagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)

Depositor

Hendrik Dietz

Article

Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

Insert

Staple sequences for a DNA origami nanorod

Use

Tags

Expression

Bacterial

Mutation

Promoter

none

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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