We narrowed to 22,864 results for: Sis

1. pEPQDKN0332

   Plasmid

   #162314

   Purpose

   Reporter plasmid for TNT SP6 Coupled Wheat Germ Extract System with detection via HiBiT

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   SP6prom-EO1-sfGFP-HiBiT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEPQD0CB0026

   Plasmid

   #162285

   Purpose

   Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEPQD0CB0246

   Plasmid

   #162286

   Purpose

   Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEPQD0CM0027

   Plasmid

   #162309

   Purpose

   Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   C-terminal tag, 9 aa linker, superfolder GFP, strep tag, stop codon
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEPQD0CM0549

   Plasmid

   #162300

   Purpose

   Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   N-terminal tag, SUMO-[TEV cleavage site]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEPQD0CM0550

   Plasmid

   #162301

   Purpose

   Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   N-terminal tag, HiBiT-SUMO-[TEV cleavage site]
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEPQD0KN0283

   Plasmid

   #162288

   Purpose

   Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEPQD0KN0282

   Plasmid

   #162287

   Purpose

   Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Prham-M.PstI-p15A-aadA

   Plasmid

   #149340

   Purpose

   expresses M.PstI DNA methyltransferase in E. coli

   Depositor

   Ichiro Matsumura

   Article

   Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

   Insert

   M.,PstI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhamnose

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEPQD0CM0539

    Plasmid

    #162306

    Purpose

    Level 0 CDS part for MoClo assembly, AATG-TTCG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    CDS, TEV protease (S219V), no stop codon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    S219V, codon optimized for E. coli

    Promoter

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEPMY0SP0002

    Plasmid

    #162304

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, 6xHis tag-[HRV 3C cleavage site]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEPQD0CM0551

    Plasmid

    #162303

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, HiBiT-[S-tag]-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPQD0CM0547

    Plasmid

    #162298

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, TrxA-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    codon optimized for E. coli

    Promoter

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEPQD0CM0546

    Plasmid

    #162297

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, MBP-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    codon optimized for E. coli

    Promoter

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPQD0CM0544

    Plasmid

    #162295

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, MBP-[Factor Xa cleavage site]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    codon optimized for E. coli

    Promoter

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEPQD0CM0543

    Plasmid

    #162294

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, GST-[TEV cleavage site]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    codon optimized for E. coli

    Promoter

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEPQD0CM0541

    Plasmid

    #162292

    Purpose

    Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    N-terminal tag, GST-[thrombin cleavage site]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    codon optimized for E. coli

    Promoter

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-HiGlpG- S116A, H196A

    Plasmid

    #158539

    Purpose

    Bacterial expression of rhomboid protease catalytic mutant HiGlpG-S116A,H196A

    Depositor

    Sinisa Urban

    Article

    Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

    Insert

    HiGlpG
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    S116A;H169A

    Promoter

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-delN-EcGlpG

    Plasmid

    #158537

    Purpose

    Bacterial expression of rhomboid protease EcGlpG without N terminus

    Depositor

    Sinisa Urban

    Article

    Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

    Insert

    delta N-terminus EcGlpG
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    N-terminal deletion (1-86)

    Promoter

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Prham-M.XbaI-p15A-aadA

    Plasmid

    #149338

    Purpose

    expresses M.XbaI DNA methyltransferase in E. coli

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al PeerJ. 2020 Sep 11;8:e9841. doi: 10.7717

    Insert

    M.XbaI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhamnose

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge