We narrowed to 7,679 results for: URE

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actc1b-mKate2-EHD1a-dEH

Plasmid

#109513

Purpose

Muscle specific zebrafish expression of mKate2 fused to EHD1a-delta-EH

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EHD1a (zgc:77049 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

dEH

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-Delta-Hairpin

Plasmid

#136030

Purpose

EIF3B (465-552) 3'UTR with the main hairpin deleted inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-1st-Hairpin

Plasmid

#136031

Purpose

EIF3B (465-552) 3'UTR with only the main hairpin inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-Disrupted-Hairpin

Plasmid

#136032

Purpose

EIF3B (465-552) 3'UTR with the main hairpin mutated inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-Restored-Hairpin

Plasmid

#136033

Purpose

EIF3B (465-552) 3'UTR with the main hairpin restored inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-EIF3B-88nt-A:U-Hairpin

Plasmid

#136034

Purpose

EIF3B (465-552) 3'UTR with the main hairpin mutated to A and U nucleotides only inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

EIF3B 3'UTR (EIF3B Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pHBDuet259

Plasmid

#121911

Purpose

NpuDnaBΔ283ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

NpuDnaBΔ283ΔC39 intein

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 283-residue endonuclease domain

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 mouse Tankyrase1 ARC2-3 (308-655)

Plasmid

#132637

Purpose

expresses mouse Tankyrase1 ARC2-3 (308-655) in E. coli

Depositor

Wenqing Xu

Article

Morrone et al Proc Natl Acad Sci U S A. 2012 Jan 31;10

Insert

Tankyrase1 (308-655)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Myc-gamma1 (in pCAGGS)

Plasmid

#118039

Purpose

GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

Depositor

Angel de Blas

Insert

GABRG1 (Gabrg1 Rat)

Tags

cMyc epitope EQKLISEEDL inserted between fourth a…

Expression

Mammalian

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pME GFP-DCX

Plasmid

#105962

Purpose

entry clone for gateway cloning

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

NP_835365.1 (DCX Human)

Tags

6xHis-thrombin cleavage site and EGFP

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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CTL2 (M2225R)

Plasmid

#96952

Purpose

Expresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coli

Depositor

Douglas Rees

Article

Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

Insert

CTL2 (M222R) of PIEZO1

Tags

6x Histidine Tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMGF130

Plasmid

#96971

Purpose

S. pombe lem2 knockout cassette with hygro resistance gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

lem2 (lem2 Fission Yeast)

Expression

Bacterial

Mutation

whole ORF deletion and replaced with hygro resist…

Promoter

N/A

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMGF172

Plasmid

#96998

Purpose

ish1 mCherry insertion cassette with ura4+ gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + ura4(…

Promoter

N/A

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTAct2 (GB0210)

Plasmid

#68191

Purpose

Provides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtAct2 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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p5'UTRAct2 (GB0193)

Plasmid

#68178

Purpose

Provides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

5'UTR AtAct2

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-Vv4CL-PhCHS-MsCHI

Plasmid

#73400

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
PhCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pPAt2S3 (GB0029)

Plasmid

#68162

Purpose

Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

At2S3 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pJBL-RNAINS4-C24A

Plasmid

#69462

Purpose

RNA-IN S4 C24A variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

Depositor

Julius Lucks

Article

Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

Insert

RNA-IN S4 with C24A mutation

Use

Synthetic Biology

Expression

Bacterial

Mutation

C24A and mutations for S4 variant (from wt)

Promoter

J23119 and J23119 (sigma 70)

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pJBL-RNAINS4-A25C

Plasmid

#69461

Purpose

RNA-IN S4 A25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

Depositor

Julius Lucks

Article

Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

Insert

RNA-IN S4 with A25C mutation

Use

Synthetic Biology

Expression

Bacterial

Mutation

A25C and mutations for S4 variant (from wt)

Promoter

J23119 and J23119 (sigma 70)

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pED55 pRS306 pRPS2-SteND-NLS-mCherry

Plasmid

#68277

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutation

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7ND NLS mCherry

Tags

mCherry

Expression

Yeast

Promoter

pRPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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