We narrowed to 150,751 results for: ins

1. RAP3-bio

   Plasmid

   #47796

   Purpose

   Expresses enzymatically monobiotinylated full-length RAP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

   Depositor

   Gavin Wright

   Article

   Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

   Insert

   Codon-optimised RAP3
   

   Tags

   enzymatic biotinylation sequence and rat Cd4 d3+4

   Expression

   Mammalian

   Mutation

   Exogenous signal peptide of mouse origin, changed…

   Promoter

   CMV

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSET a sfMatryoshCaMP6s

   Plasmid

   #100020

   Purpose

   Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   sfMatryoshCaMP6s
   

   Tags

   6x HIS tag and Xpress_EK tag

   Expression

   Bacterial

   Mutation

   cpEGFP replaced with superfolder cpGFP

   Promoter

   T7

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)

   Plasmid

   #79031

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5alpha(African green monkey pygerythrus)
   

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   March 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX Cten-SH2

   Plasmid

   #46421

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Cten (TNS4 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 444-570

   Promoter

   Tac

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. 908E

   Plasmid

   #160238

   Purpose

   TtgO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

   Depositor

   Omar Akbari

   Article

   Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

   Inserts

   Ttg Operator (2x)
   hsp70 minimal promoter
   p-element promoter
   Firefly luciferase
   Renilla luciferase
   Ubiquitin promoter
   

   Tags

   T2A GFP

   Expression

   Insect

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. 908G

   Plasmid

   #160239

   Purpose

   PipO(3x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

   Depositor

   Omar Akbari

   Article

   Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

   Inserts

   Pip Operator (3x)
   hsp70 minimal promoter
   p-element promoter
   Firefly luciferase
   Renilla luciferase
   Ubiquitin promoter
   

   Tags

   T2A GFP

   Expression

   Insect

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. 908J

   Plasmid

   #160242

   Purpose

   CymO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

   Depositor

   Omar Akbari

   Article

   Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

   Inserts

   Cym Operator (2x)
   hsp70 minimal promoter
   p-element promoter
   Firefly luciferase
   Renilla luciferase
   Ubiquitin promoter
   

   Tags

   T2A GFP

   Expression

   Insect

   Available Since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX Stat2-SH2

   Plasmid

   #46512

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Stat2 (STAT2 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 566-681

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX ShcC-SH2

   Plasmid

   #46487

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   ShcC (SHC3 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 485-594

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. P38-bio

    Plasmid

    #47727

    Purpose

    Expresses enzymatically monobiotinylated full-length P38 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

    Insert

    Codon-optimised P38
    

    Tags

    enzymatic biotinylation sequence and rat Cd4 d3+4

    Expression

    Mammalian

    Mutation

    Exogenous signal peptide of mouse origin, changed…

    Promoter

    CMV

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. CSK21_HUMAN_D0

    Plasmid

    #79707

    Purpose

    This plasmid encodes the kinase domain of CSK21. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CSK21
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pWJPrP1

    Plasmid

    #22109

    Depositor

    Susan Lindquist

    Article

    Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

    Insert

    mouse PrP ORF insert (Prnp Mouse)
    

    Use

    Construction of targeting vector

    Mutation

    mutations at aa 108 and 111 to make 3F4 epitope (…

    Available Since

    Oct. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

13. Mouse c-myc (exon I)

    Plasmid

    #8446

    Depositor

    Frederic Mushinski

    Article

    Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

    Insert

    Mouse c-myc (exon I) (Myc Mouse)
    

    Expression

    Bacterial

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

14. BRCA1-(1234-1443)

    Plasmid

    #35078

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    His

    Mutation

    Contains aa 1234-1443

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pGEX p55g(NC)-SH2

    Plasmid

    #46462

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    p55g(NC) (PIK3R3 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 56-461

    Promoter

    Tac

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX Ptk70-SH2

    Plasmid

    #46473

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Ptk70 (Srms Mouse)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 123-236

    Promoter

    Tac

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX Bks-SH2

    Plasmid

    #46406

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Bks (STAP2 Human)
    

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 141-266

    Promoter

    Tac

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX Slnk-SH2

    Plasmid

    #46502

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Slnk (Sh2d6 Mouse)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 199-323

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX Sck-SH2

    Plasmid

    #46478

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Sck/ShcB (Shc2 Mouse)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 464-573

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX Socs7-SH2

    Plasmid

    #46509

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Socs7 (SOCS4 Human)
    

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 280-386

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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