164,102 results

1. CROPseq-Guide-Puro-mCherry2

   Plasmid

   #219679

   Purpose

   CROPseq vector based on #86708 with an additional mCherry2 fluorescent gene

   Depositor

   Christoph Bock

   Article

   Boccuni et al Sci Signal. 2022 Dec 13;15(764):eabq5389

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pK18msB

   Plasmid

   #177839

   Purpose

   Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size.

   Depositor

   Christopher Johnson

   Article

   Ling et al Nat Commun. 2022 Aug 22;13(1):4925. doi:

   Type

   Empty backbone

   Use

   Bacterial gene replacement

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCpf1b-sp

   Plasmid

   #122188

   Purpose

   Bacterial genome editing plasmid with spectinomycin resistance marker and using sacB as a counter-selection marker

   Depositor

   Cheng-Cai Zhang

   Article

   Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-mGFAP-Lck-PinkFlamindo

   Plasmid

   #228394

   Purpose

   AAV vector to express the red cAMP indicator in astrocytes

   Depositor

   Tetsuya Kitaguchi

   Article

   Vittani et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

   Insert

   Pink Flamindo
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   mGFAP

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-SARS-Spike

   Plasmid

   #145031

   Purpose

   Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

   Depositor

   Fang Li

   Article

   Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

   Insert

   SARS-CoV Spike
   

   Use

   Tags

   C9 and CD5 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 16, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pTRC-APEX2

   Plasmid

   #72558

   Purpose

   Expression of cytosolic APEX2 in bacteria

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   A134P relative to APEX

   Promoter

   Available since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTW2066

   Plasmid

   #236575

   Purpose

   ISYmu1 PDS3 gRNA2 TRV2 Architecture_B

   Depositor

   Steven Jacobsen

   Article

   Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

   Insert

   ISYmu1 TnpB
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-2T PTP-1B

   Plasmid

   #8602

   Depositor

   Ben Neel

   Insert

   PTP-1B (PTPN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. cytoplasmic NAD+ biosensor

   Plasmid

   #186787

   Purpose

   NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   cytoplasmic NAD+ biosensor
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-Tomm20-HaloTag9

    Plasmid

    #175525

    Purpose

    Co-expression of HaloTag10 (nuclear) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag10 and Tomm20-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag10 = HaloTag7-Q165W and HaloTag9 = HaloTag…

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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