We narrowed to 10,873 results for: kars;

1. pSUM-GroEL-gfp

   Plasmid

   #109286

   Purpose

   Expresses gfp in mycobacteria

   Depositor

   Nicolai van Oers

   Article

   Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

   Insert

   GroEL-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GFP modified for expression in prokaryotes; inclu…

   Promoter

   GroEL

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcold-6xHis-HRV3Csite-DmLoqsPB

   Plasmid

   #41094

   Depositor

   Phillip Zamore

   Article

   Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

   Insert

   Loquacious-PB (loqs Fly)
   

   Use

   Tags

   6xHis-HRV3Csite

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pSum-LacZ-gfp

   Plasmid

   #109285

   Purpose

   Expresses gfp in mycobacteria

   Depositor

   Nicolai van Oers

   Article

   Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GFP modified for expression in prokaryotes; inclu…

   Promoter

   LacZalpha

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SLC9A3R2_PDZ_2

   Plasmid

   #103926

   Purpose

   Protein expression and purification of NHERF2 PDZ2 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   SLC9A3R2 (NHERF2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GST eIF2beta

   Plasmid

   #45900

   Depositor

   Louise Larose

   Article

   Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

   Insert

   eIF2S2 (Eif2s2 Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

6. ptagBFP-TetR-NLS-VP16

   Plasmid

   #103835

   Purpose

   Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   tagBFP-TetR-NLS-VP16 (tetR )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV2

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSV HA eIF2beta WT

   Plasmid

   #45898

   Depositor

   Louise Larose

   Article

   Kebache et al Proc Natl Acad Sci U S A. 2002 Apr 16;99

   Insert

   Eif2s2 (Eif2s2 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-RLuc8C-FHA1-NES

   Plasmid

   #138210

   Purpose

   Encodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   RLuc8C-FHA1-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-mCherry-PKAsub-RLuc8N-NES

   Plasmid

   #138211

   Purpose

   Encodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

   Depositor

   Jin Zhang

   Article

   Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

   Insert

   mCherry-PKAsub-RLuc8N-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Flag-Trib2

    Plasmid

    #131155

    Purpose

    Mammalian expression of flag-tagged mouse TRIB2

    Depositor

    Warren Pear, Karen Keeshan

    Article

    Keeshan et al Cancer Cell. 2006 Nov;10(5):401-11. doi:

    Insert

    Trib2 (Trib2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS/U6/hTID1

    Plasmid

    #13745

    Depositor

    Karl Munger

    Article

    Edwards et al Oncogene. 2004 Nov 4. 23(52):8419-31.

    Insert

    hTid1 siRNA (DNAJA3 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Inverted repeat of 21 bp with 6 nuc. spacer.

    Promoter

    Available since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pSP323 pRS306 pRPS2-Mkk2DS-NLS-mCherry

    Plasmid

    #68280

    Purpose

    SKARS: Mpk1 MAPK activity sensor: Mkk2DS (1-100) 2xNLS mCherry

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Mkk2DS NLS mCherry (MKK2 Budding Yeast)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    RPS2

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRCIscript-crhr2_TAL1A

    Plasmid

    #44236

    Depositor

    Karl Clark, Stephen Ekker

    Article

    Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

    Insert

    crhr2 pTAL scaffold 1A
    

    Use

    Mrna transcription vector

    Tags

    Expression

    Mutation

    Promoter

    T3

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRCIscript-crhr2_TAL1B

    Plasmid

    #44237

    Depositor

    Karl Clark, Stephen Ekker

    Article

    Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

    Insert

    crhr2 pTAL scaffold 1B
    

    Use

    Mrna transcription vector

    Tags

    Expression

    Mutation

    Promoter

    T3

    Available since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKEK3235

    Plasmid

    #234107

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    sfGFP
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    S30R/Y39N/N105T/Y145F/I171V/A206V

    Promoter

    pJ23100-BsaI

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKEK3220

    Plasmid

    #234099

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    sfGFP
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    S30R/Y39N/N105T/Y145F/I171V/A206V

    Promoter

    pJ23100-BsaI

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKEK3237

    Plasmid

    #234091

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    sfGFP
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    S30R/Y39N/N105T/Y145F/I171V/A206V

    Promoter

    pJ23100-BsaI

    Available since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKEK3400

    Plasmid

    #234084

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    sfGFP
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    S30R/Y39N/N105T/Y145F/I171V/A206V

    Promoter

    pJ23100-BsaI

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. rTetR-GFP-p65

    Plasmid

    #232633

    Purpose

    p65 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition

    Depositor

    Karsten Rippe

    Insert

    rTetR-GFP-p65 (RELA Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pdCas9-GFP-p65

    Plasmid

    #232630

    Purpose

    p65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacer

    Depositor

    Karsten Rippe

    Insert

    dCas9-GFP-p65 (RELA Synthetic, Human)
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHelper-CRISPRi-sgRNA

    Plasmid

    #221135

    Purpose

    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    sgRNA-scaffold with dCas9 handle
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119(SpeI)

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDEST17-NUDT5

    Plasmid

    #219955

    Purpose

    For protein purification from Ecoli

    Depositor

    Karla Feijs

    Article

    Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

    Insert

    NUDT5 (NUDT5 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDEST17-LAP3

    Plasmid

    #217661

    Purpose

    For protein purification from Ecoli

    Depositor

    Karla Feijs

    Article

    Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

    Insert

    LAP3 (LAP3 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDONR221-LAP3

    Plasmid

    #217660

    Purpose

    For gateway cloning of LAP3

    Depositor

    Karla Feijs

    Article

    Weixler et al ResearchSquare. 2024 Aug 8. doi: 10.2120

    Insert

    LAP3 (LAP3 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTRE3G-NS3/4A

    Plasmid

    #186884

    Purpose

    Doxycycline-dependent expression of NS3/4A protease gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    HCV NS3/4A protease
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTRE3G-marmoset cGAS-HA

    Plasmid

    #186864

    Purpose

    Doxycycline-dependent expression of marmoset cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Marmoset cGAS with C-terminal HA tag
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR/zeo-MACROD1

    Plasmid

    #172572

    Purpose

    For gateway cloning of N-terminal tagged MACROD1 constructs.

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pColdI-GST-mGTSF2

    Plasmid

    #215714

    Purpose

    Bacterial expression of GST-tagged GTSF2

    Depositor

    Phillip Zamore

    Article

    Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

    Insert

    GTSF2 (Gtsf2 Mouse)
    

    Use

    Tags

    6x His and GST

    Expression

    Bacterial

    Mutation

    Promoter

    CspA

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNBA001

    Plasmid

    #155323

    Purpose

    Binary vector for expression of PULSE-controlled EFR-GFP

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_(etr)8-(C120)5-PhCMVmin-EFR-GFP-T35S_RB
    

    Use

    Synthetic Biology; Eukaryotic expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNBA002

    Plasmid

    #155324

    Purpose

    Binary vector for expression of PULSE-controlled GBP-mCherry

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_(etr)8-(C120)5-PhCMVmin-GBP-mCherry-T35S_RB
    

    Use

    Synthetic Biology; Eukaryotic expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge