We narrowed to 32,144 results for: LIS;

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pcryGFP-acta1-dmdGFP

Plasmid

#172530

Purpose

alphaA-crystallin promoter (cry) drives GFP expression in the lens. The acta1 muscle-specific promoter drives expression of GFP-tagged zebrafish dmd.

Depositor

Joachim Berger

Article

Berger et al J Cell Mol Med. 2020 Jun;24(12):6680-668

Insert

dmd (dmd Zebrafish)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-43

Plasmid

#172759

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-43; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-43

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-35

Plasmid

#172755

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-35; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-35

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-19

Plasmid

#172748

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-19; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-19

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-3

Plasmid

#172740

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-3

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-12

Plasmid

#172744

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-12

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-14

Plasmid

#172745

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-14

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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MSCVpic2neo-EGFP-p16 shRNA

Plasmid

#164919

Purpose

Expresses EGFP cDNA and p16 shRNA in mammalian cells

Depositor

Ji Luo

Article

Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

Inserts

Enhanced green fluorescent protein
CDKN2A (CDKN2A Human)

Use

Retroviral

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 UPA-STOP

Plasmid

#164031

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CBX1mut-Dronpa

Plasmid

#138250

Purpose

Triple-mutant CBX1 chromodomain (residues 20-73) tagged with reversibly switchable FP Dronpa. Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

Depositor

Jin Zhang

Article

Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

Insert

CBX1mut-Dronpa

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mIsl2-EGPF

Plasmid

#153191

Purpose

Mouse Isl2 (mIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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Pmyo-3::hpo-30 gDNA (pBAB204)

Plasmid

#117385

Purpose

Allows for body-wall muscle expression of HPO-30

Depositor

Kavita Babu

Article

Sharma et al J Neurosci. 2018 Aug 8;38(32):7072-7087.

Insert

genomic DNA of hpo-30 (833 bp)

Expression

Worm

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGAL4DBD-SuUR f.l

Plasmid

#112995

Purpose

P element vector for transposon insertion of full-length Drosophila SuUR into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Sher et al Genome Res. 2012 Jan;22(1):64-75. doi: 1

Insert

SuUR (SuUR Fly)

Use

P insertion vector

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-BirA-UBE3B-Puro

Plasmid

#107351

Purpose

Expresses UBE3B fused to BirA in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

BirA-UBE3B (UBE3B Human)

Use

Lentiviral

Tags

Biotin ligase

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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hC73S-HA

Plasmid

#111529

Purpose

Expresses human KCNB1 C73S in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44.

Insert

human C73S-Kv2.1 + HA (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

C73S

Promoter

CMV

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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hC73A-HA

Plasmid

#111526

Purpose

Expresses human KCNB1 C73A in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44.

Insert

human C73A-Kv2.1 + HA (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

C73A

Promoter

CMV

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMV-TRP1

Plasmid

#65335

Purpose

standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUs

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TRP1

Use

Synthetic Biology

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-T-TEF2

Plasmid

#65340

Purpose

construction of TEF2 terminator in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2 terminator

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-P-TDH3

Plasmid

#65336

Purpose

construction of TDH3 promoter in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TDH3 promoter

Use

Synthetic Biology

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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HCKan-T-ADH1

Plasmid

#65339

Purpose

construction of ADH1 terminator in HCKan-P receiving vector

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

ADH1 terminator

Use

Synthetic Biology

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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