We narrowed to 30,068 results for: des.2
-
Plasmid#62065PurposeExpression of human MDM2 (1-120) in e. coliDepositorInsertMDM4 (MDM4 Human)
TagsGSTExpressionBacterialMutationcontains residues 1-120PromotertacAvailable SinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pv6_MECP2_MBD
Plasmid#179407PurposeCell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBDDepositorAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-4E-pEGFP-C1
Plasmid#166744PurposeExpress Venus fused to the N-terminus of the Bcl-2 family protein, Bik with mutation in BH3 domain to disrupt binding anti-apoptotic proteinsDepositorInsertBik-4E (BIK Human)
TagsVenusExpressionMammalianMutation4 hydrophobic residues in the BH3 region mutated …PromoterCMVAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE GFP-Ago2 S387E
Plasmid#60655PurposeGFP-Ago2 S387E mutant lentiviral vectorDepositorAvailable SinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS L30 Ruby3-Rab7A
Plasmid#135651PurposeRab7A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.DepositorAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs2-SH2
Plasmid#46505DepositorInsertSocs2 (SOCS2 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 28-162PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
Jam2-AP-His
Plasmid#71953PurposeExpresses the extracellular region of the JAM-2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1aL-E690Kmut_ABCA3-GFP
Plasmid#188549PurposeLentiviral vector allowing for EF1aL-driven constitutive expression of E690K mutant human ABCA3:GFP fusion proteinDepositorInsertABCA3:GFP (ABCA3 Human)
UseLentiviralTagsGFPMutationamino acid E690->K690 (ABCA3 gene)PromoterEF1aLAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1aL-W308Rmut_ABCA3-GFP
Plasmid#188550PurposeLentiviral vector allowing for EF1aL-driven constitutive expression of W308R mutant human ABCA3:GFP fusion proteinDepositorInsertABCA3 (ABCA3 Human)
UseLentiviralTagsGFPMutationamino acid W308->R308 (ABCA3 gene)PromoterEF1aLAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EZH2-mt2
Plasmid#220240PurposeExpression of an EZH2 mutant in insect cells.DepositorInsertEZH2 mt 2 (EZH2 Human)
Tags6x His, MBPExpressionInsectMutationF32A, R34A, D36A, K39A, PRKKKR494-499NAAIRSPromoterPolyhedrinAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_1-590 POLI
Plasmid#131233PurposeMammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains a 24 bp insertion within the Pol iota co…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_1-420 POLI
Plasmid#131234PurposeMammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
TagsFLAGExpressionMammalianMutationContains a 24 bp insertion within the Pol iota co…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-Nr4a1sgRNA2
Plasmid#160946PurposeGuide RNA 2 to generate Nr4a1 knockout by CRISPRDepositorAvailable SinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lrrc4-AP-His
Plasmid#71958PurposeExpresses the extracellular region of the LRRC4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
GABA(A) receptor subunit a2SE
Plasmid#49169PurposepHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit alpha-2 (GABRA2 Human)
TagsHA, bovine alpha 1 signal sequence, and pHGFP (pH…ExpressionMammalianMutationT343APromoterCMVAvailable SinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2-mt2
Plasmid#220245PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 2 (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationF32A, R34A, D36A, K39A, PRKKKR494-499NAAIRSPromoterEF-1αAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_535-1102 USP7
Plasmid#131246PurposeMammalian expression of the five USP7 UBL domains with an N-terminal Myc tag.DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationExpresses USP7 amino acids 535-1102. This truncat…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_1-205 USP7
Plasmid#131245PurposeMammalian expression of the USP7 TRAF-like domain with an N-terminal Myc tag.DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationExpresses a mutant form of our pcDNA3.1-N-Myc_1-5…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_1-560 USP7
Plasmid#131244PurposeMammalian expression of N-terminally Myc-tagged USP7, minus the UBL domainsDepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationExpresses USP7 amino acids 1 - 560. This truncati…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_535-888 USP7
Plasmid#131248PurposeMammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationA mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…Available SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only