We narrowed to 9,339 results for: mel

Showing: 6521 - 6540 of 9339 results

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pHIV-BRAFV600E-mOrange2

Plasmid

#110732

Purpose

Mammalian Expression of BRAFV600E IRES H2B-mOrange2

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

BRAFV600E IRES H2B-mOrange2 (BRAF Human)

Use

Lentiviral

Expression

Mammalian

Mutation

BRAF V600E

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pL40C_PGKintron_Cas9_Green

Plasmid

#134966

Purpose

Lentiviral vector coding Cas9 and mNeonGreen

Depositor

Beat Bornhauser

Article

Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

Insert

Cas9-P2A-mNeonGreen

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

hPGK promoter with beta-globin intron

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pLX317-CELF4

Plasmid

#115445

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

CELF4 (CELF4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgAR2

Plasmid

#167001

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 2 (AR Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgAR1

Plasmid

#167000

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 1 (AR Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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K9L mutant of H3K9me3 biosensor

Plasmid

#120807

Purpose

FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)

Expression

Mammalian

Mutation

lysine 9 is mutated to leucine 9 on histone H3

Promoter

CMV

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgAR3

Plasmid

#167002

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 3 (AR Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG CLTX-CAR

Plasmid

#157743

Purpose

knock CLTX-CAR into AAVS1 safe harbor locus for constitutive expression

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

chlorotoxin containing chimeric antigen receptor targeting glioblastoma

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Circular 200,100 (mIDUA)

Plasmid

#170123

Purpose

AAV vector carrying 2 copies of a guide RNA targeting the mouse IDUA mRNA, expressed from human and mouse U6 promoters

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Insert

Circular 200,100 guide RNA (Idua Mouse)

Use

AAV

Expression

Mammalian

Promoter

Human U6 and mouse U6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti_CRISPRi_sgAR10

Plasmid

#167003

Purpose

Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

AR Promoter sgRNA 10 (AR Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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W45A mutant of H3K9me3 biosensor

Plasmid

#120808

Purpose

FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3

Expression

Mammalian

Mutation

Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

Promoter

CMV

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pEUI(+)

Plasmid

#111834

Purpose

For mamalian expression of Ecdysone receptor-based inducible transgene expression switch.

Depositor

Hyunju Ro

Article

Lee et al Mol Ther Nucleic Acids. 2016 Sep 27;5(9)

Insert

GAL4-minimal VP16-EcR (EcR Synthetic)

Expression

Mammalian

Promoter

CMV, SV40, 10XUAS

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-2xMyc-Rac1-WT

Plasmid

#128580

Purpose

For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-EZH2-PGK-Puro-IRES-GFP

Plasmid

#75125

Purpose

Retroviral expression plasmid encoding wild type human EZH2

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pX026

Plasmid

#167153

Purpose

MoClo-compatible Level 1 (position 4) vector encoding Neonothopanus nambi luciferase nnLuz with C-terminal His-tag under control of 0.4 kb 35S promoter, for expression in plants

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

p35s-nnLuz-ocsT

Use

Luciferase

Tags

His6-tag

Expression

Plant

Promoter

p35s

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAC1-V16-eGFP

Plasmid

#122033

Purpose

AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and eGFP expression

Depositor

Liliane Tenenbaum

Article

Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

Insert

eGFP

Use

AAV

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-F-Med26-NTD

Plasmid

#49255

Purpose

Mammalian expression of flag-tagged Med26-NTD

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med 26 (MED26 Human)

Tags

Flag

Expression

Mammalian

Mutation

NTD (aa 1-339)

Available Since

Dec. 30, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5-F-Med26-R61A K62A

Plasmid

#49254

Purpose

Mammalian expression of flag-tagged human Med26 R61A K62A

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med26 (MED26 Human)

Tags

Flag

Expression

Mammalian

Mutation

R61A K62A

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pLKO.1-shCELF4-4405

Plasmid

#115466

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shCELF4-4405 (CELF4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF208

Plasmid

#101549

Purpose

Donor Vector containing ZNF208 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF208 (ZNF208 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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