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pMXs_FLAG-MCART1K91A

Plasmid

#133248

Purpose

Express FLAG-tagged MCART1K91A in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

MCART1 (SLC25A51 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells, K9…

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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PHKG1 gRNA (BRDN0001144981)

Plasmid

#76356

Purpose

3rd generation lentiviral gRNA plasmid targeting human PHKG1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PHKG1 (PHKG1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-TDAG51

Plasmid

#32699

Depositor

Richard C. Austin

Article

Hossain et al J Biol Chem. 2003 Aug 8;278(32):30317-27

Insert

human tdag51 (PHLDA1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
PGK1 gRNA (BRDN0001146969)

Plasmid

#77982

Purpose

3rd generation lentiviral gRNA plasmid targeting human PGK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PGK1 (PGK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shETV1-A

Plasmid

#74973

Purpose

shETV1 shRNA

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV1 (ETV1 Human)

Use

RNAi

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2-CRY2

Plasmid

#140572

Purpose

For use in light-induced protein inactivation through the interaction with CIB1

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-hIP-dCas9-BSD

Plasmid

#183231

Purpose

Lentiviral vectors with the human insulin promoter driving expression of dCas9, in addition to a U6-driven sgRNA

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Type

Empty backbone

Use

CRISPR and Lentiviral

Promoter

Human insulin promoter

Available Since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAGE2.0-i

Plasmid

#194639

Purpose

Empty pAGE2.0 plasmid with pBAD inducible promoter

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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STK17A gRNA (BRDN0001149480)

Plasmid

#77916

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK17A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK17A (STK17A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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STK17B gRNA (BRDN0001146764)

Plasmid

#76798

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK17B

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK17B (STK17B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-pAct-AtINTphiC31

Plasmid

#127535

Purpose

Plasmid encodes A. thaliana codon optimized Integrase phiC31.

Depositor

Martin Bonamino

Article

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

Integrase phiC31 coding sequence codon optimized for A. thaliana expression.

Expression

Plant

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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PKN3 gRNA (BRDN0001146792)

Plasmid

#77948

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN3 (PKN3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKN3 gRNA (BRDN0001146747)

Plasmid

#77949

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN3 (PKN3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKN3 gRNA (BRDN0001487150)

Plasmid

#77950

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKN3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKN3 (PKN3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pINDgw RFA-V5-EcoDam

Plasmid

#59204

Purpose

Mammalian DamID vector for fusing gene of interest with Dam-V5 using Gateway cloning

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Damid

Tags

Dam (DNA adenine methyltransferase) and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223 MERS-CoV NSP15_stop

Plasmid

#168640

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP15 (orf1ab )

Use

Other

Available Since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pGL3-Tet1 promoter A

Plasmid

#63881

Purpose

Luciferase reporter plasmid used to study transcriptional control of murine Tet1

Depositor

Kian Peng Koh

Article

Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

Insert

Tet1 promoter fragment A

Use

Luciferase

Expression

Mammalian

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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PRKAA1 gRNA (BRDN0001146526)

Plasmid

#76253

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKAA1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKAA1 (PRKAA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTol2CG NBT:NRGIIIa:polyA

Plasmid

#193012

Purpose

Tol2 transgenic construct that will express human Neuregulin1 Type IIIa in neurons

Depositor

William Talbot

Article

Lysko et al Hum Mutat. 2022 Sep;43(9):1216-1223. doi

Inserts

NBT
NRG1 type IIIa (NRG1 Human)

Use

Transposon transgenesis

Expression

Mammalian

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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ROCK2 gRNA (BRDN0001147800)

Plasmid

#76349

Purpose

3rd generation lentiviral gRNA plasmid targeting human ROCK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ROCK2 (ROCK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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