We narrowed to 14,722 results for: EGF

1. Intron-Tagging-EGFP-Frame0-Parental-Minicircle

   Plasmid

   #216124

   Purpose

   Parental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   sgRNA-SA-(GGGGS)x4-EGFP-(GGGGS)x4-SD
   

   Use

   CRISPR

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

   Plasmid

   #139997

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. M43: pHR-SFFVp-iLID::EGFP::FTH1

   Plasmid

   #122149

   Purpose

   Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FTH1 (FTH1 Human)
   

   Use

   Lentiviral

   Tags

   NLS-iLID-GFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

   Plasmid

   #185917

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8.20m-nSpRY-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. VEE-mScarlet3-IRES-EGFP-IRES-moxBFP

   Plasmid

   #242407

   Purpose

   Native (conventional) self-amplifying RNA (saRNA) control construct expresses moxBFP as visual marker of IRES functionality

   Depositor

   Ewan St. John Smith

   Article

   Lim et al bioRxiv 2024.09.24.614636

   Inserts

   mScarlet3
   IRES-EGFP
   IRES-moxBFP
   

   Use

   Self-amplifying rna

   Expression

   Mammalian

   Available Since

   Sept. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

   Plasmid

   #134858

   Purpose

   Expresses EGFP-tagged early endosome marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Ras-related protein 5A
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

   Plasmid

   #214997

   Purpose

   Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   TMEM115 (Tmem115 Mouse)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDisplay-GRAB-rDA2m-IRES-EGFP-CAAX

   Plasmid

   #208694

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2m and a membrane-localized EGFP in mammalian cells

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA2m
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast

   Plasmid

   #213117

   Purpose

   Expression of a faster-running MinE mutant in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   MinE(L4E)-EGFP (minE Escherichia coli)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   L4E

   Promoter

   EF1a

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

    Plasmid

    #79671

    Purpose

    Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

    Depositor

    Scott Sternson

    Article

    Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tol2-ISceI-E1b:EGFP;cryaa:mCherry-ISceI-Tol2

    Plasmid

    #193851

    Purpose

    E1b promoter for transgenic enhancer assays in zebrafish

    Depositor

    Junsu Kang

    Article

    Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Zebrafish expression

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

    Plasmid

    #126217

    Purpose

    Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    Gephyrin.FingR-eGFP-CCR5TC
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-Syn-PSD95.FingR-eGFP-CCR5TC

    Plasmid

    #125693

    Purpose

    Global labeling of excitatory synapses (green fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    PSD95.FingR-eGFP-CCR5TC
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11

    Plasmid

    #211942

    Purpose

    dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator)

    Depositor

    Evgeny Kvon

    Article

    Hollingsworth et al Nat Commun. 2025 Jan 6;16(1):409. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    HSP68

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid

    #185911

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV[FLEX]-CAG-EGFP-Ctrl-sponge

    Plasmid

    #235152

    Purpose

    Expresses a EGFP-based control sponge in a Cre recombinase-dependent manner

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    EGFP/control sponge
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid

    #118156

    Purpose

    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-P2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a core and U6

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJRH-1328 LV EF1a-CD20 IRES-EGFP

    Plasmid

    #201918

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD20
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

    Plasmid

    #128424

    Purpose

    Human Codon Optimized dCas9-DNMT3A3L

    Depositor

    Bradley Bernstein

    Article

    Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

    Insert

    dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    T7 Promoter

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138133

    Purpose

    Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    EGFP-anti-rabbit-IgG(Fc)-nanobody
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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