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pCD/NL-BH*deltavpu/RT-

Plasmid

#136985

Purpose

Second-generation lentiviral packaging plasmid lacking reverse transcriptase activity

Depositor

Jakob Reiser

Article

Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

Insert

HIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif

Expression

Mammalian

Mutation

carries D110E mutation in the reverse transcripta…

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pIM691

Plasmid

#74641

Purpose

alias pFA6a-ymTagBFP-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and hygromycin selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

ymTagBFP

Expression

Yeast

Promoter

none

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-R-Kras

Plasmid

#180572

Purpose

ddRFP sensor of Kras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Kras

Expression

Mammalian

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Coronin1B-pmCherryN1

Plasmid

#27694

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Coronin 1B (Coro1b Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
854-Desmocollin 1a-myc

Plasmid

#32231

Depositor

Kathleen Green

Article

Ishii et al J Invest Dermatol. 2001 Jul;117(1):26-35

Insert

Desmocollin 1a (DSC1 Human)

Tags

Myc

Expression

Mammalian

Promoter

human beta-actin

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only
pMaCTag-07

Plasmid

#124786

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeonGreen

Use

Pcr template

Tags

mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pFM571

Plasmid

#74634

Purpose

alias pFA6a-mCherry-kanMX6 - vector for C-terminal tagging of yeast genes with mCherry and G418 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

mCherry

Promoter

none

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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TRPML3-HA

Plasmid

#18832

Depositor

Craig Montell

Article

Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

Insert

TRPML3 (Mcoln3 Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
CMV-AsCas12f-WT

Plasmid

#204635

Purpose

Expression of wild-type AsCas12f in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

AsCas12f

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCMVMagFRET-1-NLS

Plasmid

#50748

Purpose

Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

Depositor

Maarten Merkx

Article

Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

Insert

gene encoding for MagFRET-1 with NLS

Expression

Mammalian

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pPD545 ZF37x6-Compact_EYFP

Plasmid

#138859

Purpose

EYFP reporter vector for the ZF37x6-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF37x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF37x6-Compact

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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PB-T3G-CERP2

Plasmid

#175498

Purpose

All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MXS_E2-Crimson

Plasmid

#62410

Purpose

MXS_chaining vector with E2-Crimson

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

E2-Crimson

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR1A-FKBP12DD C-term (w350-2)

Plasmid

#17413

Purpose

Gateway entry vector for Degron, C-term

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

FKBP12DD (L106P)

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pENTR1A-NTAP-A (w322-1)

Plasmid

#17402

Purpose

Gateway entry vector for NTAP (N-terminal affinity purification), frame A

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

CBP and SBP

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pPD536 VP16-ZF150

Plasmid

#138769

Purpose

Constitutive expression of VP16-ZF150 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF150

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD558 ZF172x6-Compact_EYFP

Plasmid

#138872

Purpose

EYFP reporter vector for the ZF172x6-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF172x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF172x6-Compact

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pPD538 VP16-ZF172

Plasmid

#138772

Purpose

Constitutive expression of VP16-ZF172 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF172

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pIM573

Plasmid

#74637

Purpose

alias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

ymTagBFP

Expression

Yeast

Promoter

none

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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MTK4_003

Plasmid

#123812

Purpose

Encodes rabbit beta-globin polyadenylation signal as a Type 4 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

rglb pA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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