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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,621 results for: crispr

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Showing: 6541 - 6560 of 25621 results

  3. pJW31

    Plasmid
    #136423
    Purpose
    Contains genes of Type I-C CRISPR-Cas system for inducible expression and chromosomal integration at attTn7
    Depositor
    Joseph Bondy-Denomy
    Publication
    Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas5
    Cas8
    Cas7
    Cas3
    Cas3
    Use
    CRISPR
    Available since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    136423-plasmid-map-sequence-id-270560

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  10. PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid
    #102891
    Purpose
    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102891-plasmid-map-sequence-id-198253

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  11. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-gs-UGI-NLS(SV40)(LbBE1.4) (RTW1293)

    Plasmid
    #114086
    Purpose
    CAG promoter expression plasmid for human codon optimized LbBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    114086-plasmid-map-sequence-id-231034

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  13. sgKRAS(B)

    Plasmid
    #85567
    Purpose
    Expresses sgRNA targeting human KRAS around codon G12
    Depositor
    Luca Grumolato
    Publication
    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRAS proto-oncogene, GTPase (KRAS Human)
    Expression
    Mammalian
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  14. sgKRAS(A)

    Plasmid
    #85566
    Purpose
    Expresses sgRNA targeting human KRAS around codon G12
    Depositor
    Luca Grumolato
    Publication
    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRAS proto-oncogene, GTPase (KRAS Human)
    Expression
    Mammalian
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  16. pAAVS1-CAG-hCAS9-neo

    Plasmid
    #166026
    Purpose
    AAVS1 targeting vector carrying CAG-SpCas9-neo
    Depositor
    Kosuke Yusa
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1-CAGCas9IRESneo
    Expression
    Mammalian
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    166026-plasmid-map-sequence-id-328056

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  18. pFETCh_ZNF219

    Plasmid
    #72367
    Purpose
    Donor vector for 3' FLAG tag of human ZNF219
    Depositor
    Eric Mendenhall, Richard M. Myers
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ZNF219 Homology Arms (ZNF219 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Available since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    72367-plasmid-map-sequence-id-132117

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  19. pFETCh_ZNF146

    Plasmid
    #72359
    Purpose
    Donor vector for 3' FLAG tag of human ZNF146
    Depositor
    Eric Mendenhall, Richard M. Myers
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ZNF146 Homology Arms (ZNF146 Human)
    Use
    CRISPR
    Tags
    3XFLAG-P2A-NeoR
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72359-plasmid-map-sequence-id-135361

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Showing: 6541 - 6560 of 25621 results