We narrowed to 14,002 results for: flag

1. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

   Plasmid

   #195505

   Purpose

   T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
   

   Use

   CRISPR; Donor vector

   Tags

   mRuby-3xFLAG-NLS-3xSV40-poly(A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)

   Plasmid

   #165456

   Purpose

   Inducible ZEB2 expression from AAVS1 locus

   Depositor

   Madeline Lancaster

   Article

   Benito-Kwiecinski et al Cell. 2021 Apr 15;184(8):2084-2102.e19.

   Insert

   ZEB2 (ZEB2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)

   Plasmid

   #139444

   Purpose

   Lentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   sequence codon-optimized

   Promoter

   SFFV

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

   Plasmid

   #104571

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
   

   Use

   CRISPR

   Tags

   NLS-3xHA-3xFLAG-4xDmrA

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT282 Lenti pEF-dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast

   Plasmid

   #187337

   Purpose

   dCas9 effector fusion for manipulating transcription

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCas9-3XFLAG-N+Z+F-T2A-tagBFP-P2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   pEF1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized

   Plasmid

   #166834

   Purpose

   Expression and purification of NLRP1 (without the PYD and CARD) from mammalian cells

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   TEV-GFP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

   Plasmid

   #185491

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

   Depositor

   Raul Perez-Jimenez, Benjamin Kleinstiver

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-eGFP

   Expression

   Mammalian

   Mutation

   D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJM529 EF1α 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA in TUPV5

   Plasmid

   #161555

   Purpose

   Constitutive expression of 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)

   Plasmid

   #104568

   Purpose

   Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain

   Depositor

   Keith Joung

   Article

   Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

   Insert

   dLbCpf1(D832A)-DmrA(x1)
   

   Use

   Tags

   3x FLAG, 3x HA, and NLS

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2)

    Plasmid

    #104569

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domains

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1(D832A)-DmrA(x2)
    

    Use

    Tags

    3x FLAG, 3x HA, and NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

    Plasmid

    #185490

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Raul Perez-Jimenez, Benjamin Kleinstiver

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET FLAG TEV cloning vector with BioBrick polycistronic restriction sites (9L)

    Plasmid

    #48288

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+8-cyp-33e1(C295A, C439A)-flag-tev-halo-his

    Plasmid

    #227158

    Purpose

    For T-REX experiments of cyp-33e1 C295A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES).

    Depositor

    Yimon Aye

    Article

    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

    Insert

    cyp-33e1 (cyp-33E1 Nematode)
    

    Use

    Tags

    Flag, HaloTag, and His6

    Expression

    Mammalian

    Mutation

    changed cysteine 295 and cysteine 439 to alanine

    Promoter

    CMV and SP6

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+8-CYP2A6(C14A, C82A, C439A)-flag-tev-halo-his

    Plasmid

    #227164

    Purpose

    For T-REX experiments of CYP2A6 C14A, C82A, and C439A triple mutant. Almost no sensing ability to reactive electrophilic species (RES).

    Depositor

    Yimon Aye

    Article

    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

    Insert

    CYP2A6 (CYP2A6 Human)
    

    Use

    Tags

    Flag, HaloTag, and His6

    Expression

    Mammalian

    Mutation

    changed cysteine 14, cysteine 82, and cysteine 43…

    Promoter

    CMV and SP6

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEJS2784 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-20a CMV-BFP

    Plasmid

    #240514

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Use

    Tags

    FLAG, hsa-miR-20 Target Sites, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEJS2785 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-125a CMV-BFP

    Plasmid

    #240515

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Use

    Tags

    FLAG, hsa-miR-125a Target Sites, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEJS2786 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-151a CMV-BFP

    Plasmid

    #240516

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Use

    Tags

    FLAG, hsa-miR-151a Target Sites, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEJS2787 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-221 CMV-BFP

    Plasmid

    #240517

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Use

    Tags

    FLAG, hsa-miR-221 Target Sites, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEJS1386 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-107 CMV-BFP

    Plasmid

    #226115

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Use

    Tags

    FLAG, hsa-miR-107 Target Sites, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEJS1350 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-92a CMV-BFP

    Plasmid

    #226109

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Use

    Tags

    FLAG, hsa-miR-92a Target Sites, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge