We narrowed to 13,831 results for: sequence
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Plasmid#64687PurposepKT vector encoding yeast-codon optimized infrared fluorescent protein iRFP.DepositorInsertiRFP
ExpressionYeastMutationNucleotide sequence has been yeast-codon optimize…Available SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LasI
Plasmid#193631PurposeConstitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.DepositorInsertLasI
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCB2-sgRNA
Plasmid#129463PurposeTemplate plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).DepositorInsertpgRNA
UseCRISPRExpressionBacterialPromoterBBa_J23119 (SpeI)Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMC-J6-dCas9-10XGP41-J9
Plasmid#202005PurposeMoClo level 0 dCas9-10XGP41 coding sequence moduleDepositorInsertdCas9-10XGP41
UseSynthetic Biology; Moclo cloning vectorTagsGS linkersAvailable SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTR47m4
Plasmid#104068PurposeLuciferase Reporter for Formaldehyde SensingDepositorInsertsxluxAB
frmR
frm Promoter m4
UseLuciferase and Synthetic BiologyExpressionBacterialMutation23r and 25th nucleotides in sequence mutated from…Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
AiRT_RTctrl
Plasmid#214070PurposeFluorescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTRDepositorInsertTurboRFP
ExpressionMammalianMutationLinker sequence present between RFP and GFPAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-Stuffer-HepmCherry
Plasmid#192825PurposeContains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoterDepositorInsertmCherry
UseCRISPR and LentiviralExpressionMammalianPromoterHepatocyte-specific promoter (HS-CRM8-TTRmin modu…Available SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL2Basic-EcadK1/EpaIMUT/EboxMUT/Ebox2MUT
Plasmid#19291DepositorInsertE-cadherin
UseLuciferaseExpressionMammalianMutationE-cad promoter sequences from -108 to +125 Ebo…Available SinceOct. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLV-mRFP1-Human UTROPHIN_1-261
Plasmid#225052PurposeExpress in mammalian cells the mRFP1 fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.DepositorInsertHuman Utrophin residues 1-261 and RFP1 (UTRN Homo Sapiens, Human)
UseLentiviralTagsHuman UTROPHIN 1-261 and mRFP1ExpressionMammalianMutationSequence is derived from ADDGENE Plasmid #26739-R…PromoterCMV promoter and enhancerAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHACK(Gal4)-DONR(T2A-LexAGAD)
Plasmid#194769PurposeTo insert T2A-LexAGAD into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-LexAGAD transgene.DepositorInsertT2A-LexAGAD
UseCRISPRExpressionInsectAvailable SinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
phRL TK 5BoxB sp 36
Plasmid#115365PurposeExpression vector to produce humanized Renilla luciferase with 5BoxB sequences in the 3'UTRDepositorInsertRenilla Luciferase
UseLuciferaseExpressionMammalianPromoterTKAvailable SinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cdk9-Halo donor
Plasmid#113113PurposeDonor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequenceDepositorInsertCdk9 donor region including homology arms and HaloTag
UseSynthetic BiologyAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B1-deltaE1-MCS
Plasmid#122558PurposeModified block 1, with deletion in E1DepositorInsertAdenovirus 5 genomic region 1-3759
UseSynthetic BiologyMutationAdenovirus sequences 481-3530 replaced with multi…Available SinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD_IPF2.0
Plasmid#163124PurposeA plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.DepositorInsertIFP2.0
TagsHisExpressionBacterialAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B6/7deltaE3
Plasmid#175748PurposeA block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6deltaE3 and pAd5-B7.DepositorInsertAdenovirus 5 genomic region 25043-35938
UseAdenoviral and Synthetic BiologyMutationAdenovirus sequences 27859-30803 replaced with Ba…Available SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB4282
Plasmid#98701PurposeA plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gata4-GFP donor
Plasmid#113115PurposeDonor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequenceDepositorInsertGata4 donor region including homology arms and eGFP
UseSynthetic BiologyAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEXP5-NT/pT7 LasR
Plasmid#193629PurposeConstitutive T7 RNAP-mediated expression of LasR transcription factor for Las quorum sensing system. LasR sequence is codon optimized for E. coli.DepositorInsertLasR
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS_36x-601_MA1+MA3+MA2
Plasmid#114364Purpose36x Widom 601 nucleosome sequenceDepositorInsert36X601-MA1_3_2
UseUnspecifiedExpressionMammalianAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAd5-B6-deltaE3
Plasmid#122559PurposeModified block 6, with deletion in E3DepositorInsertAdenovirus 5 genomic region 27859-30803
UseSynthetic BiologyMutationAdenovirus sequences 27859-30803 replaced with Ba…Available SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only