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Addgene

We narrowed to 1,284 results for: abo.1

Showing: 961 - 990 of 1284 results
  1. pKF-P14MMP2AG-T2APuroR-5h

    Plasmid
    #128256
    Purpose
    Mammalian positive feedback gene circuit in a Flp-In expression system controlling the PuroR gene. Also called mPF-PuroR.
    Depositor
    Insert
    rtTA-Advanced::P2A::EGFP::T2A::PuroR
    Use
    Synthetic Biology; Flp-in expression vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tight TRE promoter
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pGL2-HKII-A

    Plasmid
    #64513
    Purpose
    AS-30D hepatoma hexokinase II 4.3 kbp promoter-luciferase reporter vector
    Depositor
    Insert
    Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)
    Use
    Luciferase
    Tags
    firefly luciferase
    Expression
    Mutation
    Isolated from AS-30D rat hepatoma
    Promoter
    Hexokinase II
    Available since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. pGL2-HKII-C

    Plasmid
    #64515
    Purpose
    AS-30D hepatoma hexokinase II 2.1 kbp promoter-luciferase reporter vector
    Depositor
    Insert
    Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)
    Use
    Luciferase
    Tags
    firefly luciferase
    Expression
    Mutation
    Isolated from AS-30D rat hepatoma
    Promoter
    Hexokinase II
    Available since
    June 23, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. pGL2-HKII-E

    Plasmid
    #64517
    Purpose
    AS-30D hepatoma hexokinase II 0.1 kbp promoter-luciferase reporter vector
    Depositor
    Insert
    Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)
    Use
    Luciferase
    Tags
    firefly luciferase
    Expression
    Mutation
    Isolated from AS-30D rat hepatoma
    Promoter
    Hexokinase II
    Available since
    June 23, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pGL2-HKII-B

    Plasmid
    #64514
    Purpose
    AS-30D hepatoma hexokinase II 3.0 kbp promoter-luciferase reporter vector
    Depositor
    Insert
    Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)
    Use
    Luciferase
    Tags
    firefly luciferase
    Expression
    Mutation
    Isolated from AS-30D rat hepatoma
    Promoter
    Hexokinase II
    Available since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pCAGGS

    Plasmid
    #206115
    Purpose
    expression of rat Cav2.1 with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface
    Depositor
    Insert
    cacna1b (Cacna1a Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E320A, E670A, E1411A, E1707A
    Promoter
    CMV/B-actin
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pcDNA3

    Plasmid
    #206121
    Purpose
    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domains I, II, III and IV to to abolish divalent cation binding
    Depositor
    Insert
    cacna1a (Cacna1a Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E320A, E670A, E1411A, E1707A
    Promoter
    CMV
    Available since
    Oct. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. S1-Fc

    Plasmid
    #164220
    Purpose
    Expresses soluble, dimeric Fc-His fusion protein for the original SARS-CoV2 Spike S1 region, along with dTomato reporter
    Depositor
    Insert
    Spike S1 domain (S SARS-CoV)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. Tet-pLKO-puro

    Plasmid
    #21915
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2009
    Availability
    Academic Institutions and Nonprofits only
  10. RBD-Fc

    Plasmid
    #164221
    Purpose
    Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter
    Depositor
    Insert
    Spike RBD (S SARS-CoV)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
  11. Tet-pLKO-neo

    Plasmid
    #21916
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2009
    Availability
    Academic Institutions and Nonprofits only
  12. pgRNA_CV2

    Plasmid
    #167165
    Purpose
    pgRNA_CV2 is derived from gRNA_cloning vector (Addgene plasmid ID: 41824) by adding about 80 bps from the sgRNA sequence as well as an AgeI site. In the literature, sgRNA_AL is used as an alias.
    Depositor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
  13. pET28a-RBP-GFP(S45C)-ZdK1

    Plasmid
    #185791
    Purpose
    Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker
    Depositor
    Insert
    RBP-sfGFP(S45C)-ZdK1
    Use
    Tags
    10xHis, TEV protease cleavage site after His Tag
    Expression
    Bacterial
    Mutation
    Ser to Cys mutation at start of GFP, residue 45 i…
    Promoter
    T7
    Available since
    July 22, 2022
    Availability
    Academic Institutions and Nonprofits only
  14. pGL2-HKII-D

    Plasmid
    #64516
    Purpose
    AS-30D hepatoma hexokinase II 1.0 kbp promoter-luciferase reporter vector
    Depositor
    Insert
    Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)
    Use
    Luciferase
    Tags
    firefly luciferase
    Expression
    Mutation
    Isolated from AS-30D rat hepatoma
    Promoter
    Hexokinase II promoter
    Available since
    June 23, 2015
    Availability
    Academic Institutions and Nonprofits only
  15. pGL2-HKII-F

    Plasmid
    #64518
    Purpose
    AS-30D hepatoma hexokinase II 0.075 kbp exon I-luciferase reporter vector
    Depositor
    Insert
    Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)
    Use
    Luciferase
    Tags
    firefly luciferase
    Expression
    Mutation
    Isolated from AS-30D rat hepatoma
    Promoter
    Hexokinase II promoter deleted
    Available since
    June 23, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 961 - 990 of 1284 results