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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 816 results for: NUMB

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Showing: 641 - 660 of 816 results

  1. pETDuet-solo-NHis6-NocO-H279A

    Plasmid
    #240406
    Purpose
    expresses N-His tagged NocO H279A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, His…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240406-plasmid-map-sequence-id-478117

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  2. pETDuet-solo-NHis6-NocO-E94A

    Plasmid
    #240401
    Purpose
    expresses N-His tagged NocO E94A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240401-plasmid-map-sequence-id-478116

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  5. pToxAmp1

    Plasmid
    #218285
    Purpose
    Amplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanism
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
    Expression
    Yeast
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218285-plasmid-map-sequence-id-433687

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  6. pS44i8GH-1

    Plasmid
    #207525
    Purpose
    Conditionally-replicating in Pseudomonas vector, high-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Article
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Insert
    oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP;
    Use
    CRISPR; Pseudomonas vector
    Available Since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207525-plasmid-map-sequence-id-409596

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  7. pB6-Id1-IRES-loxP-NeoR-loxP-DTA-TK

    Plasmid
    #158616
    Purpose
    B6J Id1-LSL-DTA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Id1 (Id1 Mouse)
    Use
    Cre/Lox and Mouse Targeting
    Expression
    Mammalian
    Mutation
    Insertion of IRES-loxP-NeoR-loxP-DTA after the st…
    Available Since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    158616-plasmid-map-sequence-id-333472

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  20. pSP6-RNAP

    Plasmid
    #48143
    Purpose
    Shuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage SP6 under control of the xylose inducible promoter PxylA
    Depositor
    Dieter Jahn
    Article
    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2
    Insert
    rna polymerase SP6
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    V314A (numbering based on NCBI Sequence ID: NP_85…
    Available Since
    Oct. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
    48143-plasmid-map-sequence-id-373044

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Showing: 641 - 660 of 816 results