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We narrowed to 23,591 results for: Sele

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Showing: 641 - 660 of 23591 results

  8. ATP1A1_G4_Q118R_Std_Dual_epegRNA_tevopreQ1

    Plasmid
    #187454
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187454-plasmid-map-sequence-id-367517

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  9. pY036_ATP1A1_G5_Array

    Plasmid
    #86620
    Purpose
    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via hdr using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86620-plasmid-map-sequence-id-168857

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  11. pY036_ATP1A1_G3_Array

    Plasmid
    #86619
    Purpose
    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86619-plasmid-map-sequence-id-168855

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  13. ATP1A1_G6_T804N_Std_Dual_epegRNA_tevopreQ1

    Plasmid
    #187457
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N pegRNA (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187457-plasmid-map-sequence-id-367465

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  15. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86613-plasmid-map-sequence-id-168851

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  16. ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1

    Plasmid
    #187459
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187459-plasmid-map-sequence-id-367469

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  18. ATP1A1_plasmid_donor_RD

    Plasmid
    #86551
    Purpose
    Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 (ATP1A1 Human)
    Use
    CRISPR and TALEN; Co-selection via hdr using ouab…
    Expression
    Mammalian
    Mutation
    Q118R, N129D
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86551-plasmid-map-sequence-id-171421

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  19. pDRM18 LTN (Luciferase tdTomato Neo)

    Plasmid
    #174721
    Purpose
    Expresses the firefly luciferase gene, E2A, tdTomato, P2A, and the neomycin resistance gene.
    Depositor
    Wayne Tilley
    Article
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    tdTomato
    aph
    Use
    Lentiviral and Luciferase
    Tags
    E2A linker and P2A linker
    Expression
    Mammalian
    Promoter
    MND, MND (off Luc-E2A), and MND (off Luc-E2A-tdTo…
    Available Since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    174721-plasmid-map-sequence-id-349021

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  20. pDGB3omega1_KanR_BastaR

    Plasmid
    #186426
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Use
    Synthetic Biology; Binary vector for escherichia …
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186426-plasmid-map-sequence-id-369066

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Showing: 641 - 660 of 23591 results