Addgene

We narrowed to 2,002 results for: rp1

Showing: 641 - 660 of 2002 results

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pSLIK 3xFLAG-KPNA2 zeo

Plasmid

#192330

Purpose

Lentiviral expression vector for an inducible 3xFLAG-KPNA2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNA2 (KPNA2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-KPNA2

Plasmid

#192306

Purpose

Gateway entry vector for an inducible 3xFLAG-KPNA2

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNA2 (KPNA2 Human)

Use

Gateway entry vector

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB DPP9L

Plasmid

#166788

Purpose

Insect cell expression vector for DPP9L (protein purification)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

HIS-TEV

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB DPP9S

Plasmid

#166787

Purpose

Insect cell expression vector for DPP9S (protein purification)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

HIS-TEV

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-KLF3

Plasmid

#49100

Purpose

Expresses KLF3 in mammalian cells

Depositor

Merlin Crossley

Article

Bell-Anderson et al Diabetes. 2013 Aug;62(8):2728-37. doi: 1

Insert

Krüppel-Like Factor 3(KLF3)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only
pFastBac HTB DPP9S S730A

Plasmid

#169946

Purpose

Purification of catalytically-dead DPP9S from insect cells

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

HIS-TEV

Expression

Insect

Mutation

S730A

Promoter

polyhedrin

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB DPP9S D808N

Plasmid

#169947

Purpose

Purification of catalytically-dead DPP9S from insect cells

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Use

Tags

HIS-TEV

Expression

Insect

Mutation

D808N

Promoter

polyhedrin

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pET41sIII_GST-ALC1-K77R

Plasmid

#174813

Purpose

Bacterial expression of inactive ALC1 (K77R disrupts ATP binding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

ALC1-K77R (CHD1L Human)

Use

Tags

GST

Expression

Bacterial

Mutation

K77R

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET41sIII_GST-ALC1-E175Q

Plasmid

#174814

Purpose

Bacterial expression of inactive ALC1 (E175Q disrupts ATP binding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

ALC1-E175Q (CHD1L Human)

Use

Tags

GST

Expression

Bacterial

Mutation

E175Q

Promoter

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R459E

Plasmid

#164024

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R459E

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R464E

Plasmid

#164025

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R464E

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C E485R

Plasmid

#164026

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, E485R

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C E490R

Plasmid

#164027

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, E490R

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R495E

Plasmid

#164028

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R495E

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C D511K

Plasmid

#164029

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, D511K

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C Y527A

Plasmid

#164030

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, Y527A

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_KPNA2_Arm

Plasmid

#109866

Purpose

Protein expression and purification of KPNA2_Arm

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

KPNA2_Arm (KPNA2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-RNF68 [dRING]

Plasmid

#126549

Purpose

Expresses FLAG-HA-tagged RNF68 [dRING mutant] in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF68 (PCGF1 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

RING domain deletion mutant [dRING mutation is a …

Promoter

LTR

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-RNF68 [Y252D]

Plasmid

#126550

Purpose

Expresses FLAG-HA-tagged RNF68 [Y252D] mutant in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF68 (PCGF1 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

Y252D point mutant

Promoter

LTR

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-Eef2TOPm-RLCP-HCVIRES-FL

Plasmid

#233629

Purpose

Expression of bicistronic reporter with mutated TOP motif

Depositor

Shintaro Iwasaki

Article

Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

Insert

Promoter and 5'UTR of Eef2 gene with mutation in TOP motif and HCV IRES (Eef2 Mouse)

Use

Luciferase

Tags

Expression

Mutation

AGAAGG in TOP motif

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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