We narrowed to 6,862 results for: GFP expression plasmids

1. pAT9750-BEAR-mCherry

   Plasmid

   #162990

   Purpose

   BEAR target plasmid with split mCherry and disrupted 5' splice site

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   mCherry split with an intron between amino acids 119-120
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAT-9222

   Plasmid

   #124222

   Purpose

   Cloning plasmid for EGxFP assay with self-targeting gRNA-s

   Depositor

   Ervin Welker

   Article

   Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

   Insert

   self-targeting gRNA between EGFP halves
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd

   Plasmid

   #216170

   Purpose

   Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- FOXA.bs- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

   Plasmid

   #216172

   Purpose

   Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- Control- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-sgRNA EF1Alpha-puro-T2A-BFP

   Plasmid

   #60955

   Purpose

   Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-EF1a-hMLH1dn-Hygro

   Plasmid

   #195512

   Purpose

   Lentiviral expression plasmid of hMLH1dn with P2A-HygroR marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   hMLH1dn-P2A-HygroR (MLH1 Human)
   

   Use

   Lentiviral

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Human MLH1 del754-756

   Promoter

   EF-1a

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

   Plasmid

   #172318

   Purpose

   CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKII-GAL4-iLight-VP16

   Plasmid

   #170274

   Purpose

   NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

   Insert

   NLS-Gal4(1-65)-iLight(human codon optimized)-VP16
   

   Use

   AAV

   Tags

   Expression

   Mutation

   See Comments

   Promoter

   CaMKII

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGMKOSiE

   Plasmid

   #20865

   Depositor

   Keisuke Kaji

   Article

   Kaji et al Nature. 2009 Mar 1. ():.

   Insert

   myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Myc Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLJM1-FIRE-pHLy

    Plasmid

    #170775

    Purpose

    Ratiometric biosensor expressed with CMV promoter for probing lysosomal pH in mammalian cells

    Depositor

    Aimee Kao

    Article

    Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry and mTFP1

    Expression

    Mammalian

    Mutation

    84 nucleotide Signal Sequence switched to the beg…

    Promoter

    CMV

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Linked Free ClLIS1 (M)

    Plasmid

    #182487

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS(M)-LS
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmCherry C1 Omp25

    Plasmid

    #157758

    Purpose

    Expression of mcherry-labelled mitochondrial outer membrane protein 25 in cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    SYNJ2bp (SYNJ2BP Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. MCP-MSN

    Plasmid

    #210704

    Purpose

    This plasmid express EF1a promoter driven MCP-MSN transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. MCP-eN3x9

    Plasmid

    #210706

    Purpose

    This plasmid express EF1a promoter driven MCP-eN3x9 transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. MCP-NMS

    Plasmid

    #210705

    Purpose

    This plasmid express EF1a promoter driven MCP-NMS transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MCP-3x9aaTAD

    Plasmid

    #210707

    Purpose

    This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTE4938

    Plasmid

    #107527

    Purpose

    Expresses Fn crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Fn crRNA
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and human U6

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Linked Free NES

    Plasmid

    #182485

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Linked Free PcPTS

    Plasmid

    #182486

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS-PTS
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTE4561

    Plasmid

    #107525

    Purpose

    Expresses Lb crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Lb crRNA
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and human U6

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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