We narrowed to 13,494 results for: Mpl;

1. pCDNA3-biotin-myc- mCE

   Plasmid

   #82475

   Purpose

   Expresses mRNA capping enzyme containing N terminal biotin and myc tag

   Depositor

   Daniel Schoenberg

   Article

   Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d

   Insert

   Biotin (Rngtt Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_6HT-TBP6.7(WT)

   Plasmid

   #112720

   Purpose

   Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(WT)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_6HT-TBP6.7(P46A)

   Plasmid

   #112721

   Purpose

   This mutation (P46A) marks the first residue within the evolved region of U1A.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P46A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P46A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a_6HT-TBP6.7(Q48T)

   Plasmid

   #112725

   Purpose

   This mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q48T)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q48T

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a_6HT-TBP6.7(P51A)

   Plasmid

   #112728

   Purpose

   Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P51A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P51A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a_6HT-TBP6.7(Q54A)

   Plasmid

   #112730

   Purpose

   Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q54A)
   

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q54A

   Promoter

   T7

   Available Since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-Flag-Sesn-C mSesn2 (delAB)

   Plasmid

   #111799

   Purpose

   Mammalian Expression of mSesn2

   Depositor

   Jun Hee Lee

   Article

   Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

   Insert

   mSesn2 (Sesn2 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AB domain truncation

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-Flag-Sesn-B+C mSesn2 (delA)

   Plasmid

   #111798

   Purpose

   Mammalian Expression of mSesn2

   Depositor

   Jun Hee Lee

   Article

   Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

   Insert

   mSesn2 (Sesn2 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   A domain truncation

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-Flag-Sesn-A mSesn2 (delBC)

   Plasmid

   #111797

   Purpose

   Mammalian Expression of mSesn2

   Depositor

   Jun Hee Lee

   Article

   Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

   Insert

   mSesn2 (Sesn2 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   BC domain truncation

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGS_N_Strep

    Plasmid

    #101651

    Purpose

    Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, KpnI linker, Twin-Strep tag
    

    Tags

    Twin-Strep tag

    Expression

    Insect

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGS_C_Strep

    Plasmid

    #101652

    Purpose

    Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    LRP6 Signal peptide, MCS linker, Twin-Strep tag
    

    Tags

    Twin-Strep tag

    Expression

    Insect

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMflCT-o4

    Plasmid

    #101313

    Purpose

    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.

    Depositor

    Sébastien Rodrigue

    Article

    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7

    Inserts

    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    cat resistance gene
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-HA-REDD1 N181-211 (#595)

    Plasmid

    #99939

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delAA181-211insNAAIRS linker

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-HA-REDD1 N187-207 (#596)

    Plasmid

    #99940

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delAA187-207insNAAIRS linker

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-HA-REDD1 85-232 (#427)

    Plasmid

    #99922

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal deletion; encodes AA 85-232

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HA-REDD1 101-232 (#183)

    Plasmid

    #99923

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal deletion; encodes AA 101-232

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-HA-REDD1 1-192 (#421)

    Plasmid

    #99925

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C-terminal deletion; encodes AA 1-192

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-HA-REDD1 1-209 (#430)

    Plasmid

    #99926

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C-terminal deletion; encodes AA 1-209

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-HA-REDD1 96-110 (#189)

    Plasmid

    #99929

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Internal deletion of AA 96-110

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-HA-REDD1 111-135 (#190)

    Plasmid

    #99930

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Internal deletion of AA 111-135

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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