We narrowed to 18,339 results for: Mut

1. pcDNA5 FRT TO Myc ArC Long WT

   Plasmid

   #59809

   Purpose

   Allows the integration of Myc ArC Long in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

   Insert

   Aurora C (AURKC Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBp-FGFR2b-K660N

   Plasmid

   #45707

   Depositor

   Matthew Meyerson

   Article

   Liao et al Cancer Res. 2013 Jun 20.

   Insert

   FGFR2 IIIb (FGFR2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   K660N

   Promoter

   Available Since

   June 24, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-mCherry-TSC2(AA)-NLS

   Plasmid

   #192443

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A mutations to block Akt phosphorylation; targeted to the nucleus.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A)-NLS (TSC2 Human)
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.2-Cx43-siResist

   Plasmid

   #49852

   Purpose

   Encodes human connexin 43 with wobble mutations conferring resistance against an siRNA

   Depositor

   Robin Shaw

   Article

   Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

   Insert

   Connexin 43 (GJA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains several wobble mutations to confer resis…

   Promoter

   CMV

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pGEX6P1-Ubl-sr1(SIRPT1)

   Plasmid

   #75298

   Purpose

   Expresses Sacsin Ubl-sr1 (SIRPT1 repeat) domain (1-339) in fusion with GST (bacterial)

   Depositor

   Kalle Gehring

   Article

   Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

   Insert

   Human Sacsin 1-339
   

   Use

   Tags

   GST-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVC228 VEGF Site#3

   Plasmid

   #47507

   Purpose

   human gRNA expression vector targeting VEGF

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-VEGF site 3 (VEGFA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF1a-nSpCas9-NG

   Plasmid

   #171692

   Purpose

   Expresses nSpCas9-NG in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   nSpCas9-NG
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…

   Promoter

   EF1a

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-p27 T187A Luc

   Plasmid

   #23048

   Depositor

   William Kaelin

   Article

   Zhang et al Nat Med. 2004 Jun . 10(6):643-8.

   Insert

   p27 (CDKN1B Human)
   

   Use

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   T187A: mutation at codon 187 changing from T (ACG…

   Promoter

   Available Since

   Jan. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ165

   Plasmid

   #109327

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoter

   Depositor

   Yiping Qi

   Article

   Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

   Insert

   zCas9
   

   Use

   CRISPR; Gateway compatible zcas9 entry clone

   Tags

   3x FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   Promoter EC1.2 enhancer fused to EC1.1 promoter

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLPCX-Cx43-FS-IRES-GFP

    Plasmid

    #65438

    Purpose

    Mammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral production

    Depositor

    Trond Aasen

    Article

    Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

    Insert

    Cx43 (GJA1 Human)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    frameshift (FS) mutation 35delA

    Promoter

    CMV

    Available Since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRVdGL-1Cre

    Plasmid

    #98038

    Purpose

    Second-generation rabies viral vector genome

    Depositor

    Ian Wickersham

    Article

    Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

    Insert

    mCre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRVdGL-4EGFP

    Plasmid

    #98037

    Purpose

    Second-generation rabies viral vector genome

    Depositor

    Ian Wickersham

    Article

    Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. iBE3 CLYBL

    Plasmid

    #174569

    Purpose

    doxycycline inducible BE3 expression for human CLYBL locus knock-in

    Depositor

    Mathew Garnett

    Article

    Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

    Insert

    BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet ON

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-SFFV-RAB10 T23N-WPRE-UbC-Emerald

    Plasmid

    #203802

    Purpose

    Lentiviral vector plasmid expressing human RAB10 mutation T23N (dominant negative mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    RAB10 (RAB10 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    T23N

    Promoter

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJS699

    Plasmid

    #168779

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD (S betacoronavirus)
    

    Use

    Tags

    Cmyc

    Expression

    Mutation

    N343Q

    Promoter

    GAL1

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDF1-ADAMTS18 Wt

    Plasmid

    #29552

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Wild type

    Promoter

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

17. Hy_pMT Flag-Hel25E K91N

    Plasmid

    #110121

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    K91N mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Hy_pMT Flag-Hel25E D193E

    Plasmid

    #110120

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    D193E mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA3_shRNA resistant PKA-Cα-mEGFP

    Plasmid

    #114143

    Purpose

    Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAS_4xMx PylT C41CA_FLAG_Mx PylRS

    Plasmid

    #140013

    Purpose

    Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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