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STT3A pmRFP (663)

Plasmid

#61997

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

STT3A (STT3A Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
kiCAP-AAV-MyoAAV4A

Plasmid

#224448

Purpose

Rep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDYNSL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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PB-GFP-Gal8

Plasmid

#127191

Purpose

Piggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.

Depositor

Jordan Green

Article

Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

Insert

GFP-Gal8 (LGALS8 Human)

Tags

Gal8 is fused to the c-terminus of GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV2A

Plasmid

#224440

Purpose

Rep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDQTTL insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-flex-taCasp3-TEVp

Plasmid

#45580

Depositor

Nirao Shah, Jim Wells

Article

Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

Has Service

AAV1, AAV5, and AAV8

Inserts

Caspase 3 (CASP3 Human, Synthetic)
Tobacco Etch Virus protease

Use

AAV; Adeno associated viral vector

Mutation

Linker replaced with a TEV protease cleavage site

Promoter

EF1a

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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TERT - WT

Plasmid

#213827

Purpose

Expresses human telomerase reverse transcriptase, the catalytic subunit of telomerase

Depositor

Coleman Lindsley

Article

Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

Insert

human TERT (TERT Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet-responsive promoter PTight, consisting of sev…

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUDP082

Plasmid

#103875

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus

Use

CRISPR

Expression

Yeast

Promoter

ScTDH3

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-Dsup-deltaC

Plasmid

#90023

Purpose

To express GFP-tagged Dsup lacking C-terminal region in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

Insert

Dsup

Tags

AcGFP1

Expression

Mammalian

Mutation

Deletion of C-terminal region

Promoter

CMV

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-Cas9-T2A-EGFP-ires-puro

Plasmid

#78311

Purpose

Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

Depositor

Timo Otonkoski

Article

Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

Insert

WT SpCas9-T2A-EGFP-ires-puromycin resistance

Use

CRISPR

Tags

T2A-EGFP-ires-puro

Expression

Mammalian

Promoter

CAG

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_WT

Plasmid

#81737

Purpose

Gateway Donor vector containing PTEN , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PRKCA_WT

Plasmid

#82225

Purpose

Gateway Donor vector containing PRKCA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKCA (PRKCA Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV1A

Plasmid

#224437

Purpose

Rep/Cap plasmid for the production of MyoAAV 1A, a muscle-tropic AAV capsid in mice.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDLTTP insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUDP002

Plasmid

#103872

Purpose

Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Inserts

hph
Spcas9 D147Y P411T

Use

CRISPR

Tags

NLS

Expression

Yeast

Mutation

D147Y P411T

Promoter

Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-uvsY

Plasmid

#163914

Purpose

Expresses uvsY for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsY (uvsY Enterobacteria phage T4)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

Plasmid

#139093

Purpose

Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

dxCas9-NG-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pUDP013

Plasmid

#103873

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha

Use

CRISPR

Expression

Yeast

Promoter

ScTDH3

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_VHL_WT

Plasmid

#81874

Purpose

Gateway Donor vector containing VHL , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

VHL (VHL Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MyoAAV4E

Plasmid

#224452

Purpose

Rep/Cap plasmid for the production of MyoAAV 4E, a muscle-tropic AAV capsid in mice and macaques.

Depositor

Pardis Sabeti

Article

Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RGDFNNT insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-MmACE2 (Mouse)

Plasmid

#158087

Purpose

Expresses mouse ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

Mouse ACE2 (Ace2 Mouse)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-FOXL2

Plasmid

#192914

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of FOXL2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

FOXL2 (FOXL2 Human)

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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