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pDONR223-NEK5

Plasmid

#23434

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK5 (NEK5 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pCS2-Prom1-TEVp-TwinStrep-His / IRES2 / Pcdh21-TEVp-Myc-Flag

Plasmid

#210820

Purpose

Mammalian overexpression vector for polycistronic co-expression of C-terminally Strep and His tagged human Prom1s1 (WT) and C-terminally Myc and Flag tagged human Pchd21 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Inserts

Prominin 1 (PROM1 Human)
Protocadherin 21 (CDHR1 Human)

Tags

Myc, 3xFlag and TwinStrep, 10xHis

Expression

Mammalian

Promoter

CMV and CMV / IRES2

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-TALEN_HD (SPC right)

Plasmid

#126695

Purpose

right TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

human SPC right

Use

TALEN

Tags

NLS, truncated N term and TAL, FokI

Expression

Mammalian

Mutation

R451P in SPC

Promoter

EF1

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a-TALEN_NN (SPC left)

Plasmid

#126694

Purpose

left TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

human SPC left

Use

TALEN

Tags

0.5 repeat NN, TAL, FokI and NLS, truncated N ter…

Expression

Mammalian

Promoter

EF1

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag NEK3

Plasmid

#20547

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

NEK3 (NEK3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-NEK3

Plasmid

#23821

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK3 (NEK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-VRK2

Plasmid

#23534

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

VRK2 (VRK2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag NEK11

Plasmid

#20546

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

NEK11 (NEK11 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-VRK3

Plasmid

#23687

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

VRK3 (VRK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-myc-AP1S3 F4C

Plasmid

#58293

Purpose

mammalian expression of AP1S3 F4C mutant

Depositor

Francesca Capon

Article

Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

Insert

AP1S3 (AP1S3 Human)

Tags

6xHis and myc

Expression

Mammalian

Mutation

F4C: c.11T>G, p.Phe4Cys

Promoter

CMV

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-myc-AP1S3 R33W

Plasmid

#58294

Purpose

mammalian expression of AP1S3 R33W mutant

Depositor

Francesca Capon

Article

Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

Insert

AP1S3 (AP1S3 Human)

Tags

6xHis and myc

Expression

Mammalian

Mutation

R33W: c.97C>T,p.Arg33Trp

Promoter

CMV

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pDONR223-NEK4

Plasmid

#23497

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK4 (NEK4 Human)

Use

Gateway donor vector

Mutation

Short isoform, only goes to aa632

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NEK11

Plasmid

#23816

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK11 (NEK11 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-SNRK

Plasmid

#23405

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SNRK (SNRK Human)

Use

Gateway donor vector

Mutation

A290G,C346G

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag VRK2

Plasmid

#20663

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

VRK2 (VRK2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
Atg7_L (OZ557)

Plasmid

#28082

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting Atg7 (atg7 Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
Atg7_R (OZ558)

Plasmid

#28083

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting Atg7 (atg7 Zebrafish)

Use

Zebrafish targeting

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
pDONR P2R-P3-IRES_ECFP

Plasmid

#48354

Purpose

Contributes an IRES-ECFP cassette as the 3’-module during MultiSite Gateway cloning of a bi-cistronic mRNA shared with a two-part fusion protein encoded by the 5′- and middle modules.

Depositor

Anna Planas, Tomas Santalucia

Article

Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

Insert

IRES_ECFP

Use

Gateway entry vector

Mutation

ECFP translation is mediated by an IRES. Contains…

Promoter

none

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-NEK10

Plasmid

#23437

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK10 (NEK10 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

Plasmid

#235307

Purpose

Lentiviral expression of ComMAND open-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Lentiviral and Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

TRE3G (3rd-generation Tet system)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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