obtaining full Cas9 RNP

lentiviral expression of c-Myc

Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA (#154868). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

Mammalian expression of mScarlet

For the protein expression with ALFA tag at C-terminus in mammalian cells

Bacterial expression of M-MLV reverse transcriptase

lentiviral expression of human POLQ

Gateway destination vector

Expresses a functionally inactive version of ARNT with 5’ 414 bp deletion [Δ414] in mammalian cells.

LacZ, pNBU1 backbone with BsaI sites, AmpR, ErmR

Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

Expresses SP-Cas9 protein in bacterial cells

Fluorescent reporter for calmodulin 1

inducible lentiviral expression of PHF2