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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,896 results for: 110

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Showing: 6641 - 6660 of 11896 results

  1. MKC86_HBA1(tEPOR-2A-YFP)

    Plasmid
    #232405
    Purpose
    AAV production plasmid for tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at HBA1 locus using HBA1-sg4 gRNA. HBA1 UTRs flank full tEPOR-2A-YFP cassette. Homology arms mediate full HBA1 replacement.
    Depositor
    Kyle Cromer, Matthew Porteus
    Article
    Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552
    Inserts
    Truncated Erythropoietin Receptor (EPOR Human)
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Use
    AAV
    Expression
    Mammalian
    Available Since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232405-plasmid-map-sequence-id-462759

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  3. pCS2-NES-Myc(340-439)-GFP-IRES-mCherry (NES-DBD)

    Plasmid
    #231237
    Purpose
    MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NES for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Expression
    Mammalian
    Mutation
    NES fused to the DNA-binding-domain of MYC (resid…
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231237-plasmid-map-sequence-id-459172

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  4. pCS2-MYC(Δ1-143; Δ340-439)-GFP-IRES-mCherry (ΔTADΔDBD)

    Plasmid
    #231235
    Purpose
    MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and DNA-binding-domain (residues 340-439) for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Expression
    Mammalian
    Mutation
    TAD deleted (delta1-143), DBD deleted (delta340-4…
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231235-plasmid-map-sequence-id-459171

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  5. pCS2-NLS-Myc(340-439)-GFP-IRES-mCherry (NLS-DBD)

    Plasmid
    #231236
    Purpose
    MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NLS for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Expression
    Mammalian
    Mutation
    NLS fused to the DNA-binding-domain of MYC (resid…
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231236-plasmid-map-sequence-id-459169

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  9. pD649-HAsp-LRIG2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156976
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    LRIG2 (LRIG2 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156976-plasmid-map-sequence-id-303061

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  10. pD649-HAsp-DNER-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156926
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    DNER (DNER Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    156926-plasmid-map-sequence-id-302961

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  13. Human/mouse ELL2

    Plasmid
    #127254
    Purpose
    Expresses a human/mouse chimera that increases ELL2 expression in mammalian cells
    Depositor
    Christine Milcarek
    Article
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Insert
    N-terminal human/C-terminal mouse ELL2 (ELL2, Ell2 Mouse, Human)
    Use
    Other
    Tags
    His6X
    Expression
    Mammalian
    Mutation
    N terminal amino acids (1-287) are human, C termi…
    Promoter
    pEF1a
    Available Since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    127254-plasmid-map-sequence-id-250057

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Showing: 6641 - 6660 of 11896 results