We narrowed to 11,361 results for: ENA
-
Plasmid#215153PurposeExpresses gRNA targeting CCND1 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.DepositorInserthSpCas9
UseCRISPRTags3XFLAG and GFPExpressionMammalianPromoterCbhAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
FokI(inactive)-mCherry-LacI-NLS
Plasmid#215100PurposeExpresses FokI(inactive)-mCherry-LacI-NLS in mammalian cells.DepositorInsertFokI(inactive)-mCherry-LacI-NLS
ExpressionMammalianMutationFokI(inactive) mutant D69A.PromoterCMVAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A
Plasmid#22023DepositorInsertmVenus-PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
UseRetroviralExpressionMammalianMutationThe LOV domain contains the mutation C450A.Rac1 s…Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
Parvalbumin scFv [L114/3]
Plasmid#182072PurposeMammalian Expression of Parvalbumin scFv. Derived from hybridoma L114/3.DepositorInsertrecombinant mouse scFv targeting Parvalbumin (Rattus norvegicus) (Pvalb Mouse)
TagsHA, HisExpressionMammalianPromoterCMVAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-P3-Alb-Rosmarinus
Plasmid#183462PurposeTo produce AAV with the P3 promoter to express Alb(mouse)-rosmarinus fusion proteinDepositorAvailable SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
GIV-FLAG S245A (SA)
Plasmid#86095PurposeExpression GIV in mammalian cellDepositorAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-331-3p
Plasmid#103444PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-331-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-331-3p target (MIR331 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro-BAP1-C91S-HA
Plasmid#154021PurposeMammalian Expression of HA-tagged p.C91S Mutant BAP1DepositorInsertBAP1 (BAP1 Human)
UseRetroviralTagsHemagglutinin (HA)ExpressionMammalianMutationp.C91S (Missense Mutation, Catalytically Inactive)Available SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRC55-GFP-DHX37
Plasmid#192150Purposeexpress GFP-DHX37DepositorAvailable SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-P3-Alb-mCarmine
Plasmid#183464PurposeTo produce AAV with the P3 promoter to express Alb-mCarmine fusion proteinDepositorAvailable SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pan-Neurofascin scFv [L11A/41]
Plasmid#206722PurposeMammalian Expression of Pan-Neurofascin scFV. Derived from hybridoma L11A/41 scFv.DepositorInsertPan-Neurofascin (Rattus norvegicus) recombinant scFV (Nfasc Mouse)
ExpressionMammalianPromoterCMVAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBGE3.0
Plasmid#165988PurposeMulti-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Nm/Kan resistance.DepositorInsertsRK2/RP4 origin of transfer
repA1B1C1
nourseothricin N-acetyl transferase
neomycin resistance
UseSynthetic Biology; Bacterial and yeast cloning; c…Available SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBSWHhc-p53
Plasmid#133903PurposePositive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWHDepositorAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75236PurposeCRISPR/Cas9 plasmid against human NFATc1DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-mtagBFP-2A STINGHAQ V155M
Plasmid#102592PurposeLentivector to express STING V155M HAQ and mTagBFPDepositorInsertSTING (STING1 Human)
UseLentiviralExpressionMammalianMutationV155M R71H-G230A-R293QPromoterSFFVAvailable SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hH4-AVITEV
Plasmid#74051Purposeretroviral expression plasmid for human histone H4 with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman Histone-H4 (H4C16 Human)
UseRetroviralTagsAVI-TEVExpressionMammalianPromoterpMSCV-LTRsAvailable SinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Recruited polymerase expression - pCMV-T7-ePhi29(+exo)-CC(N5) (LM2982)
Plasmid#208972PurposeRecruited ePhi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.DepositorInsertePhi29(+exo)-BPNLS-CC(N5)
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…PromoterCMV and T7Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
H517 SONIC HRASV12 donor
Plasmid#138177PurposeCRISPR SONIC: HRAS G12V donor plasmidDepositorInsertHRASV12 (HRAS Human)
UseNhej donorAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-485-3p
Plasmid#103553PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-485-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-485-3p target (MIR485 Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)
Plasmid#75237PurposeCRISPR/Cas9 plasmid against human NFATc1DepositorInsertsgRNA against NFATc1 (NFATC1 Human)
UseCRISPRAvailable SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only