We narrowed to 14,602 results for: RON

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pAAV Jph3gRNA mEmerald hSyn mTagBFP2-CAAX2

Plasmid

#236246

Purpose

Endogenous tagging of Junctophilin 3 with mEmerald with a fluorescent marker for the plasma membrane

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

gRNA for rat Jph3, mEmerald donor and mTagBFP2-CAAX2

Use

AAV

Tags

mTagBFP2

Promoter

U6 and human Synapsin1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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Reckleen_3_multieditdropout

Plasmid

#233458

Purpose

Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

Depositor

Anke Becker

Article

Elsayed et al Commun Biol. 2025 Oct 30;8(1):1509. doi:

Insert

Level 2 RECKLEEN plasmid, Ptac lambda Red operon, Ptet SpG cas9, J23117 acrIIA4

Use

CRISPR

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX--DmrB-DmrB-Ripk3(deltaC)-2A-mCherry-puro

Plasmid

#231973

Purpose

Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

Ripk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)

Use

Lentiviral

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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peGFP NOVA2 op

Plasmid

#224354

Purpose

Express GFP-tagged human NOVA2 (codon optimized)

Depositor

Nicolas Charlet-Berguerand

Article

Mattioli et al Am J Hum Genet. 2020 Apr 2;106(4):438-45

Insert

NOVA2 (NOVA2 Human)

Tags

eGFP

Expression

Mammalian

Mutation

codon optimized for expression in human cells

Promoter

CMV and T7

Available Since

Sept. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

Plasmid

#216251

Purpose

Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

Cas9 and sgSEC61B.N1 (SEC61B Human)

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-ARNTL-shRNA1

Plasmid

#166913

Purpose

Lentivirus for inducible knockdown of human ARNTL(BMAL1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

ARNTL shRNA (BMAL1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-ARNTL-shRNA2

Plasmid

#166914

Purpose

Lentivirus for inducible knockdown of human ARNTL(BMAL1)

Depositor

Roland Friedel

Article

Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

Insert

ARNTL shRNA (BMAL1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)

Plasmid

#160623

Purpose

TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:dCas9:VPR-Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)

Plasmid

#160627

Purpose

TU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domains

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35s-MS2:TV-Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pET20b_Pa-PiuA

Plasmid

#128955

Purpose

expresses Pseudomonas aeruginosa PiuA in E. coli

Depositor

James Naismith

Article

Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

Insert

iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)

Tags

6xHis and pelB leader

Expression

Bacterial

Mutation

Signal peptide sequence has been removed

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30-roGFP1-iE

Plasmid

#83312

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

Insert

roGFP1-iE

Tags

6xHIS

Expression

Bacterial

Mutation

ro1(C48S/S147C/Q204C) plus X insertion, iE betwe…

Promoter

T5

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT/TO Plk4 KD ∆24-mCherry

Plasmid

#80272

Purpose

mammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deleted

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

mCherry

Expression

Mammalian

Mutation

deletion of multi-phospho degron (amino acids 282…

Promoter

CMV

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 nt 400-1782 delta440-500 delta1449-1735

Plasmid

#60633

Purpose

ZKSCAN1 circular RNA expression plasmid containing minimal sufficient introns

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pEBG Jip3 (nterm)

Plasmid

#58942

Purpose

interacts with MAPK signaling module

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

Insert

mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)

Tags

GST

Expression

Mammalian

Mutation

deleted aa 271-1817

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only
Human Wild-type ASAP1

Plasmid

#235212

Purpose

Expresses Wild-Type ASAP1 in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al Proc Natl Acad Sci U S A. 2018 May 1;115

Insert

ASAP1 (ASAP1 Human)

Tags

HA

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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TDH3pro-rtTA-tetO7pro-T7pol-pRS413

Plasmid

#239293

Purpose

Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system

Depositor

Anita Hopper

Article

Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

Inserts

T7 polymerase with NLS and P278L
reverse tetracycline transactivator (rtTA)

Expression

Yeast

Mutation

Contains M2-SE-G72P mutations, described in Addge…

Promoter

TDH3 and tetracycline-responsive element (TRE) an…

Available Since

June 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

Plasmid

#214265

Purpose

Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.

Depositor

D. James Surmeier

Insert

Flag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

N/A

Promoter

Synapsin

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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PB-CAG-ChR2-YFP

Plasmid

#204345

Purpose

PiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.

Depositor

Ivo Lieberam

Article

Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

Insert

ChR2-YFP

Tags

Yellow fluorescent protein

Expression

Mammalian

Mutation

C-terminus (aa315-737) is deleted - PMID: 1461559…

Promoter

CAG

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG-CLTX CD32a-tm CD3z CAR

Plasmid

#171963

Purpose

Donor plasmid for CLTX T-CAR expression in human cells

Depositor

Xiaoping Bao

Article

Chang et al Nat Commun. 2023 Apr 20;14(1):2266. doi:

Insert

Synthetic CLTX CD32a-tm CD3z CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-353)-GCN4-L-mCherry

Plasmid

#193715

Purpose

Expression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353)

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-353)-GCN4-L-mCherry (KIF5B Human, Synthetic)

Tags

mCherry

Expression

Mammalian

Mutation

hsKIF5B(aa1-353); mCherry: Met1 Deleted

Promoter

Chicken beta-actin

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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